猪源肺炎克雷伯菌的分离鉴定及其耐药性分析  

Isolation,identification and drug resistance analysis of Klebsiella pneumoniae from swine

作　　者：王羽[1] 董文龙[1] 王巍[1] 张喜庆[1] 李雪嵩 马红霞[1] 高云航[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医科学》2017年第12期1570-1576,共7页Chinese Veterinary Science

摘　　要：通过菌落形态观察、生化试验及16S rDNA基因序列测定对分离菌株进行鉴定,共获得7株猪源肺炎克雷伯菌,将其命名为Kp1~Kp7。致病性试验结果表明,7株分离菌株对小鼠均具有较强的致病性。药敏试验结果显示,分离菌株的耐药表型差异性较大,对强力霉素、大观霉素及阿米卡星的耐药性较强。对分离菌株整合子的检测表明,仅能从Kp1检测到消毒剂-磺胺基因(qac EΔ1-sul1)、整合酶基因(IntⅠ)以及基因盒。本研究为肺炎克雷伯菌引起的疾病选择合理的抗菌药物提供了一定的科学依据,并为进一步研究其耐药机制奠定了基础。Total 7 Klebsiella pneumoniae isolated strains were identified by morphological characteristics,biochemical test and 16S rDNA sequencing,and named Kpl to Kp7. inimal pathogenicity test showed that all the isolated strains had strong pathogenicity to mice. The results of antimicrobial susceptibility test showed that the resistance phenotypes of isolated strains were different,and the isolated strains were highly susceptible to doxycycline,spectinomycin and amikacin. The integrin detection of the isolated strains showed that the qacE △ 1-sull,Int I and gene cassette were only detected in Kpl. This study provided a scientific basis for the selection of reasonable antimicrobial agents against diseases caused by K pneumoniae,and laid a foundation for further study of its drug resistance mechanism.

关 键 词：肺炎克雷伯菌 整合子 分离鉴定 耐药性分析 

分 类 号：S852.612[农业科学—基础兽医学]