金黄色葡萄球菌生物被膜形成的鉴定及白果外种皮多糖对其抑制作用的研究  

Identification of Staphylococcus aureus in biofilm formation and inhibition of polysaccharide from Ginkgo biloba sarcotesta on biofilm

作　　者：徐子恒 王宏军 栾祖香 蒋红 王培 王金莉 

机构地区：[1]锦州医科大学,辽宁锦州121001

出　　处：《中国兽医科学》2017年第12期1602-1608,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(KPS调控鸡免疫缺陷活性成分的结构解析及分子机制;31201951);锦州医科大学校长基金-奥鸿博泽研究生科研创新基金项目(白果外种皮多糖对金葡菌生物被膜抑制作用的研究;AH2015003)

摘　　要：为体外鉴定金黄色葡萄球菌生物被膜的形成,研究新鲜白果外种皮多糖(GBSP)对金黄色葡萄球菌生物被膜的抑制作用。通过刚果红鉴定、结晶紫定量检测考察金黄色葡萄球菌在体外建立生物被膜,并用微孔平板法检测多糖对金黄色葡萄球菌早期和成熟期生物被膜的最小抑膜浓度(MBIC)和最小杀膜浓度(MBBC)。结果显示,金黄色葡萄球菌可以产生多糖黏附素(PIA);金黄色葡萄球菌生物被膜随着培养时间的延长,生物被膜逐渐成熟,在12 h左右开始黏附载体表面,24 h之后达到成熟期;新鲜GBSP对金黄色葡萄球菌早期生物被膜的MBIC和MBBC是100 mg/mL。表明,新鲜GBSP对金黄色葡萄球菌生物被膜具有良好的体外抑制作用,为新鲜GBSP开发为抗菌制剂奠定了理论基础。To study the inhibitory effects of polysaccharides from fresh Ginkgo biloba sarcotesta （GBSP） on the biofilm of Staphylococcus aureus. METHODS To identify S. aureus bacteria can establish biofilms in vitro by Congo red and crystal violet. To detecte the MBIC and MBBC of the early and mature biofilm of S. aureus by the method of microtubule-plate. RESULTS The S. aureus could produce PIA. As the incubation time prolonged,the S. aureus biofilm began to mature gradually. Especially,it began to adhere on the surface of the carrier after 12 h and reached maturity after 24 h. TheMBIC and MBBC of the inhibition by fresh GBSP on early S. aureus biofilm were 100 mg/mLrespectively. The fresh GBSP has an inhibition effect on S. aureus biofilm in vitro,and it can provide the theoretical basis for the development of antibacterial agent.

关 键 词：金黄色葡萄球菌 生物被膜 抑制作用 新鲜白果外种皮 多糖 

分 类 号：S852.611[农业科学—基础兽医学]