针刺对支气管哮喘大鼠肺组织PI3K蛋白表达的影响  

Regulation of Acupuncture on PI3K Expression in The Lung Tissues of Asthma Rats

作　　者：杨金华 赵叶 李双 韩君萍 虞跃跃 樊志忠 崔建美 

机构地区：[1]华北理工大学,河北唐山063000

出　　处：《中国中医基础医学杂志》2017年第11期1602-1604,1614,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81303046)-ERK1/2通路对哮喘大鼠气道重塑;气道炎症的调控与针刺干预的分子机制研究

摘　　要：目的:探讨针刺对哮喘大鼠肺组织中PI3K蛋白表达的影响。方法:将40只SPF级雄性SD大鼠按随机数字表法分为空白组、模型组、针刺组和阻断剂组各10只,各组分别给予相应干预后用生理记录仪检测大鼠肺功能的变化,用Western Blot法和免疫组织化学法检测大鼠肺组织PI3K蛋白的表达。结果:呼吸功能测定显示模型组大鼠肺顺应性降低,肺弹性阻力升高,较空白组、针刺组和阻断剂组改变明显;western Blot和免疫组化法检测示模型组PI3K蛋白表达较空白组、针刺组、阻断剂组升高;针刺后PI3K蛋白表达降低,阻断剂组与空白组比较差异无统计学意义。结论:针刺可降低大鼠肺组织中PI3K蛋白表达以减轻气道重塑,改善和恢复肺功能。Objective： To examine the mechanism of acupuncture on PI3K expression in the lung tissues of asthmatic rats.Methods： 40 SPF male SD rats were randomly divided into 4 groups with 10 rats in each group.Groups were given accordingly interventions.Physiological recorder is used to inspect the pulmonary function changes,western blot and immunohistochemical method were employed to observe the protein expression of PI3K in the lung tissue of rats.Results：The lung compliance of the asthma model group are decreased with the pulmonary elastic resistance increased comparing with the blank group,acupuncture group and blocking group.Western Blot and immunohistochemical method,The protein expression of PI3K was higher in asthmatic model group than other groups.but there was no statistical difference among the blocking group,the acupuncture group and the blank group.Conclusion： Acupuncture can reduce the protein expression of PI3K in lung tissue of rats to reduce airway remodeling and improve lung function.

关 键 词：针刺 哮喘 PI3K 

分 类 号：R245.9[医药卫生—针灸推拿学]