葡萄转录因子数据库中17个MADS-box成员在果实发育成熟过程中的表达特征分析  

Expression characteristic analysis of MADS-box family members of grape transcription factors in berry development and ripening process

作　　者：崔梦杰 王晨[1] 吴伟民[2] 王洁 汤崴[1] 张文颖[1] 朱旭东[1] 贾海锋[1] 沈文彪 房经贵[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院,南京210014 [3]南京林业大学林学院,南京210037 [4]南京农业大学生命科学学院,南京210095

出　　处：《果树学报》2017年第12期1497-1508,共12页Journal of Fruit Science

基　　金：江苏省科技自主创新基金;中国博士后特别资助(2014T70533);国家博士后基金面上项目(2013M531373)

摘　　要：【目的】了解17个MADS-box转录因子成员在葡萄果实发育成熟过程中的调控作用。【方法】利用Plantcare软件分析了葡萄类型转录因子的启动子元件,预测其与果实发育、成熟过程相关的潜在作用;以二倍体欧亚种葡萄品种‘魏可’为试材,采用qRT-PCR技术分析了17个MADS-box成员在葡萄果实发育成熟过程中8个重要时期的时空表达模式,初步鉴定参与葡萄果实发育成熟调控的重要成员;果实转色前7 d通过ABA、NAA处理进一步验证其在葡萄果实发育与成熟中的作用。【结果】MADS-box成员的启动子均含有与果实发育、成熟相关的motif作用元件,且其含有的元件个数存在差异,表明其可能在调控葡萄果实发育与成熟过程中功能存在冗余,同时各自的作用可能存在一定的差异;qRT-PCR分析显示,所有成员均在葡萄果实发育成熟过程中表达,且在不同时期呈现差异表达;其中,VvAG和Vv FBP24在果实硬核期高表达,而在果实转色和成熟中几乎不表达,表明其可能参与果实生长发育的正调控;而VvMADS1、VvMADS9、VvSVP-like1和Vv AP3只在果实转色与成熟过程中高表达,说明它们可能促进了果实的转色与成熟;ABA在果实转色特定时期上调了MADS-box家族6个成员的表达,而NAA下调其表达;另一方面,NAA上调了MADS-box家族8个成员的表达,相反ABA下调其表达,表明这些成员可能响应ABA/NAA调控葡萄果实发育与成熟过程。【结论】葡萄转录因子MADS-box的17个成员均参与了果实发育过程的调控。其中,6个成员可能参与了葡萄果实的成熟过程的正调控,而另外8个成员可能促进了葡萄果实的生长发育从而抑制了果实的转色与成熟过程。[Objective] Fruit development and maturation are important stages for fruit quality formation. Fruit ripening regulation is valuable for commercial production of grape. MADS-box Transcription fac- tors are a class of proteins which directly or indirectly interact with specific cis acting function in promot- er region, and regulate the initiation of gene transcription. However, its function may vary greatly with different family members and regenerate different regulation modes during grape berry, non-climacteric fruit, development and ripening. In this study, the regulation function of 17 MADS-box transcription factors during ＇Wink＇ grape berry development and maturation were investigated. [Methods] The potential functions of MADS-box transcription factor members in the processes of grape development and matura- tion were predicted by analyzing their promoter motif elements using the Plantcare software. The variety ＇Wink＇ （＇Muscat Bailey A＇＇Rubel Muscat X＇） was used as experimental material. The berry samples were collected in eight development stages. ABA and NAA were sprayed one week before color change stage. The fruit size, firmness, content of soluble solids, glucose, sucrose, and fructose were measured. We analyzed the different spatial and temporal expression modes of various transcription factor members in eight developmental stages of grape berry to preliminarily screen the vital members participating in berry development process and verify the regulation function acting importantly in coloration and ripening process of grape berry. [Results] Promoters of MADS-box members contained elements related to grape berry development and ripening and the different number of each type of elements, indicating that they participated in regulating berry development and ripening process, although their function were different. MADS-box members expressed in berry development and exhibited different expression modes in different stages. VvAG and VvFBP24 had high expression level only in hard stone st

关 键 词：葡萄 转录因子 荧光定量PCR 启动子元件 果实发育与成熟 

分 类 号：S663.1[农业科学—果树学]