基于液滴微流控芯片技术的抗白念珠菌药物筛选研究  

Study on droplet microfluidic chips for drug screening against Candida albicans

作　　者：郑振[1,2] 陈阳 李武宏[1] 朱臻宇[1] 洪战英[1] 柴逸峰[1] 

机构地区：[1]第二军医大学药学院 [2]解放军91837部队卫生队

出　　处：《药学学报》2017年第12期1884-1889,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81373376)

摘　　要：本文发展了一种用于筛选抗白念珠菌药物的液滴微流控芯片技术,自行制备了液滴生成芯片,利用阿尔玛蓝在活细胞存在下能显示荧光信号的特性,将其作为指示剂和药物、白念珠菌一起包裹在液滴中,能快速检测候选化合物的抗真菌活性。以4种抗真菌药物两性霉素B、卡泊芬净、5-氟胞嘧啶、特比萘芬和新药艾迪康唑为研究对象,分别进行抗白念珠菌活性分析,并与常规的96孔板法进行比较。结果表明,液滴芯片方法孵育2 h即可实现抗真菌药物活性的快速分析,所测得的最小抑菌浓度值与96孔板法的一致率为100%,而且试剂消耗非常少。本文建立的液滴微流控芯片法简便快速,适用于大量抗真菌药物的快速筛选。A droplet microfluidic chip system was developed for drug screening against Candida albicans. The microfluidic chip was designed and prepared for the formation of droplets. Alamar blue was selected as an indicator for its characteristic of fluorescence mission in live cells. Four antifungal drugs （amphotericin B, caspofungin, 5-fluorocytosine, terbinafine） and a new drug （iodiconazole） were selected as model drugs to test the microfluidic chip approach. At the same time, 96-well microplate method was performed to verify the applicability of the chip method. The results showed that the developed droplet microfluidic chip platform was able to complete the antifungal susceptibility test within 2 h. In comparison with the 96-well microplate method, the microfluidic chip method showed a consistence of 100% with regard to the minimum inhibition concentrations and less reagent consumption. The new droplet microfluidic chip method is simple, rapid and suitable for rapid screening of antifungal drugs.

关 键 词：液滴微流控芯片 阿尔玛蓝 抗真菌药物筛选 白念珠菌 艾迪康唑 

分 类 号：R917[医药卫生—药物分析学]