基于UHPLC-MS/MS代谢组学技术的不同采收期黄芩质量比较研究  

Comparative study on quality of Scutellaria baicalensis Georgi in different harvest periods using UHPLC-MS/MS metabolomics technology

作　　者：庞溢媛 薛立英[1,2] 郑艳红[1,2] 周玉枝[1] 秦雪梅[1] 杜冠华[1,3] 张翔[1,4] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]中国医学科学院药物研究所,北京100050 [4]美国路易斯维尔大学,肯塔基州路易斯维尔40292

出　　处：《药学学报》2017年第12期1903-1909,共7页Acta Pharmaceutica Sinica

基　　金：山西省科技基础条件平台建设项目(2014091022);山西省科技攻关项目(20140313008-14);山西省重点技术创新项目[晋财建-(2013)99号];山西省重点研发计划项目(201703D321023-3)

摘　　要：黄芩的质量与采收期有着密切的关系。本文以一年生和两年生的不同采收期黄芩为研究对象,采用UHPLC-MS/MS代谢组学手段检测其化学成分种类和含量的动态变化,寻找且指认不同采收期的差异代谢物并分析其含量变化,最后进行差异代谢物间的相关性分析。结果显示,不同采收期的黄芩化学成分存在明显差异,并呈现规律性的变化。据此,若以黄芩素等黄酮苷元类成分为目标成分,则建议采收时间为5月;若以黄芩苷等黄酮苷类成分为目标成分,则建议最佳采收时间在7~8月。因此,基于UHPLC-MS/MS的植物代谢组学方法可用于不同采收期黄芩的化学成分分析,为黄芩药材确定最佳采收期提供参考,也为现有中药材质量评价方法提供一种新的思路。The quality of Scutellaria baicalensis Georgi has a close relationship to the harvest time.With the annual and biennial Scutellaria baicalensis Georgi in different harvest periods as samples,we detected the dynamic changes of chemical compositions in their contents by UHPLC-MS/MS metabolomics technology,then identified the biomarkers of different harvest periods and analyzed their changes in content,finally analyzed the correlation among different metabolites.The results showed that chemical compositions of different harvest periods were obviously different,and showed regular changes.According to the results,if the flavonoid aglycones are chosen as the target components,the proposed harvest time could be May.If flavonoid glycosides,the time is from July to August.Plant metabolomics methodology based on UHPLC-MS/MS can be used to detect the chemical compositions of Scutellaria baicalensis Georgi in different harvest periods,which provide a reference to the optimum harvest period of Scutellaria baicalensis Georgi,and also provide a new method of quality evaluation of traditional Chinese medicinal materials.

关 键 词：黄芩 UHPLC-MS MS 代谢组学 采收期 

分 类 号：R917[医药卫生—药物分析学]