牛卵母细胞及着床前胚胎发育的蛋白质组学研究进展  

Advances on the Proteomics of Bovine Oocyte and Preimplantation Embryo Development

作　　者：濮黎萍 陈富美[1] 赵秀玲[1] 王焕景[1] 候振 徐壮壮 张鹏飞[1] 张明[1] 

机构地区：[1]广西大学动物繁殖研究所亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《生物技术通报》2017年第11期54-59,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31460603);广西水牛遗传繁殖重点实验室开放课题(SNKF-2015-02)

摘　　要：蛋白质组学作为一种能够全景式展示特定生物学过程的蛋白质表达谱的高通量研究手段,正被应用到越来越多的研究领域。牛的卵子发生以及着床前胚胎的生长发育都离不开蛋白质的一系列变化,通过蛋白质组学的研究手段可对牛卵母细胞成熟以及胚胎发育分子机制进行研究。前人通过构建成熟前后的卵母细胞或不同时期的着床前胚胎等的蛋白质组表达谱,来获得与卵母细胞成熟相关的标志蛋白,并对这些标志物进行验证和分析,将有利于理解牛卵母细胞成熟、体外受精及着床前胚胎发育的分子机制,为提高牛卵母细胞的成熟率以及胚胎的发育率及提高牛繁殖率等奠定基础。主要从双向聚丙烯酰胺凝胶电泳、色谱技术以及质谱技术等蛋白质组学研究手段为切入点,对蛋白质组学在牛卵母细胞和着床前胚胎发育过程中的研究进展进行综述,为进一步研究牛卵母细胞及附植前胚胎提供参考。Proteomics,as a high-throughput method for displaying protein expression profiles of specific biological processes ina panoramic view,is being applied in more and more research fields. Bovine oogenesis and development of preimplantation embryo areinseparable from a series of changes in protein,thus the molecular mechanism of bovine oocyte maturation and embryo development can beinvestigated by proteomics. By establishing the proteomic expression profiles of oocytes before and after maturation as well as preimplantationembryos in different stages,we obtained marker proteins related to oocyte maturation and further validated and analyzed these markers,benefiting to understand the molecular mechanism of bovine oocyte maturation,in vitro fertilization and preimplantation embryo development. Itlays a foundation for improving the mature rate of bovine oocyte,the rate of embryonic development and the rate of bovine reproduction. In thispaper,the progress of proteomics in the bovine oocytes and development of preimplantation embryos was reviewed from the methods of studyingproteomics,such as two-dimensional polyacrylamide gel electrophoresis,chromatography and mass spectrometry. To provide reference forfurther study of bovine oocyte and preimplantation embrv.

关 键 词：蛋白质组 卵母细胞 着床前胚胎 

分 类 号：S823[农业科学—畜牧学]