生长激素释放肽-2对自发性高血压大鼠心肌纤维化的抑制作用  

GHRP-2 Attenuates Cardiac Fibrosis in Spontaneously Hypertensive Rats

作　　者：张鹏[1] 朱元杰 闫莉[1] 谷靖丽 曹济民[1] 

机构地区：[1]中国医学科学院基础医学研究所/北京协和医学院基础学院生理学系,100005

出　　处：《医学研究杂志》2017年第11期16-19,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81670313,81300139)

摘　　要：目的研究生长激素释放肽-2(GHRP-2)是否可抑制心肌纤维化。方法由于自发性高血压大鼠(SHR)存在自然发生的严重心肌纤维化,本研究利用SHR作为心肌纤维化模型。SHR从12周龄开始给予GHRP-2皮下注射治疗(100μg/kg,每日1次,共4周),同龄Wistar大鼠皮下注射等量生理盐水作为正常对照组;利用苦味酸天狼猩红染色法显示SHR左心室肌Ⅰ型胶原和Ⅲ型胶原,用Van Gienson染色法显示左心室总胶原水平;用羟脯氨酸含量测定法评估心肌中胶原合成水平。结果与正常对照组相比,未经处理的SHR左心室肌中Ⅰ型和Ⅲ型胶原明显增多,羟脯氨酸含量也显著增高。GHRP-2治疗可明显降低SHR心肌中Ⅰ型、Ⅲ型胶原和总胶原的水平,并降低羟脯氨酸含量。结论 GHRP-2可以通过抑制胶原合成而减轻SHR左心室纤维化。Objective To investigate whether growth hormone releasing peptide -2 （GHRP -2） could attenuate cardiac fibrosis in spontaneously hypertensive rats （ SHR）. Methods SHR shows severe cardiac fibrosis and were used as a model of cardiac＇ fibrosis in this study. SHR were treated with GHRP- 2 （100μg/kg, se, qd） for 4 weeks starting from the 12th week of birth. Pierosirius red staining was used to visualize type Ⅰ and type Ⅲ collagens, Van Gienson staining was applied to visualize total eollagens, and hydroxyproline as- say was used to quantitatively evaluate the synthetic level of myocardial collagen. Results Compared with control Wistar rats, GHRP -2 treatment significantly decreased the levels of type Ⅰ and type Ⅲ collagens and the total collagens, and also reduced the level of hydroxyproline. Conclusion GHRP - 2 attenuates cardiac fibrosis via suppressing cardiac synthesis of collagens.

关 键 词：生长激素释放肽 心肌纤维化 自发性高血压大鼠 胶原 

分 类 号：R3[医药卫生—基础医学]