牛化脓隐秘杆菌病肾脏Caspase-3、Caspase-8、Caspase-9、Bax和Bcl-2的表达  

Expression of Caspase-3,Caspase-8,Caspase-9,Bax and Bcl-2 in kidneys of cattle infected with Arcanobacterium pyogenes

作　　者：范春玲[1] 胡丹[1] 兰明慧[1] 郭东华[1] 原冬伟[1] 刘琳珊[1] 张国华[1] 朱战波[1] 周玉龙[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2017年第12期2266-2269,2274,共5页Chinese Journal of Veterinary Science

基　　金：黑龙江省教育厅科学技术研究资助项目(12541582)

摘　　要：通过研究牛化脓隐秘杆菌感染牛肾脏组织的病理学变化及检测Caspase-3、Caspase-8、Caspase-9、Bax和Bcl-2的表达,来确定牛肾脏组织的坏死和细胞凋亡情况。本研究首先筛检5头自然感染化脓隐秘杆菌病牛和2头阴性健康对照牛,采集其肾脏组织样本。采用H.E.染色法观察病理组织学变化;采用免疫组织化学方法来检测肾脏组织中与细胞正负凋亡有关的调节基因Bax和Bcl-2的表达情况以及凋亡通路蛋白Caspase-3、Caspase-8和Caspase-9的表达情况。结果显示,感染牛肾脏组织有坏死,间质有中性粒细胞浸润;感染组牛肾脏肾小球内皮细胞和肾小管上皮细胞Bax、Caspase-3和Caspase-9表达呈阳性,与对照组相比,差异显著;有少量肾小管上皮细胞、肾小球内皮细胞Caspase-8和Bcl-2表达呈阳性,但与对照组相比差异不显著。结果表明,化脓隐秘杆菌能够引起牛肾脏组织实质细胞发生坏死和凋亡,且以线粒体凋亡途径为主。This experiment was conducted to determine whether Arcanobacterium pyogenes （A. pyogenes） could cause bovine kidneys necrosis and their parenchymal cells apoptosis by studying the pathological change and the expression of Caspase-3 ,Caspase-8 ,Caspase 9 ,Bax and Bcl 2 of the bovine kidneys infected with A. pyogenes. Specimens of bovine kidneys from 2 healthy dairy cow and 5 cattle infected with A. pyogenes were selected. Methods of H. E. and immunohistochemistry were exploited to research the histopathologic changes and investigate the expression of Caspase-3, Caspase 8, Caspase-9, Bax and Bcl-2 of the kidneys. Bax and Bcl-2 are two regulative genes related to cell apoptosis in positive and negative aspect, respectively. Caspase-3, Caspase-8 and Caspase 9 are associated proteins in apoptosis pathway. The results showed that the infective kidneys ex pressed necrosis,with their matrix infiltrated by neutrophils. Bax, Caspase-3 and Caspase-9 were expressed in the renal glomerular endothelial cells and renal tubular epithelial cells in the infected kidneys. There were few expression of Caspase 8 and Bel-2 in the infected kidneys. The number of positive expressed cells of Bax,Caspase 3 and Caspase-9 was greater in the infected group than that in the control group （P〈0.05）. No significant difference of the expression of Caspase 8 and Bcl 2 was found between two groups （P〈0. 05）. The results suggested that A. pyogenes could cause the parenchymal cells of the host kidneys necrosis,apoptosis and the mitochondria signaling pathways dominated in the pathogenesis of A. pyogenes.

关 键 词：牛 化脓隐秘杆菌 肾脏 坏死 凋亡 

分 类 号：S852.3[农业科学—基础兽医学] S855.99[农业科学—兽医学]