糖尿病小鼠骨骼肌FoxO1及PPARγ的表达及意义  

Expression and significance of FoxO1 and PPARγ in the skeletal muscle of KKAy mice

作　　者：廖鑫[1] 陈其荣[1] 阳琰[1] 高琳[1] 邓凡曲 张晗[1] 张凌 牟芝群 

机构地区：[1]遵义医学院附属医院内分泌科,贵州遵义563003 [2]遵义医药高等专科学校护理系

出　　处：《中国老年学杂志》2017年第23期5759-5761,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.81660142);贵州省社会发展攻关项目[SY(2013)3033]

摘　　要：目的观察KKAy糖尿病小鼠骨骼肌组织中叉头状因子(Fox)O1、过氧化物酶体增殖物激活受体(PPAR)γ的表达,探讨FoxO1的表达与糖脂代谢的关系。方法8周龄KKAy小鼠8只为糖尿病模型组(DM),予以高脂饲料喂养;8周龄C57BL/6J小鼠16只,随机分为空白对照组(NC)8只、高脂喂养组(HFD)8只。喂养至16 w后检测3组小鼠空腹血糖(FBG)、胰岛素(Ins)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C);采用实时荧光定量PCR(RT-PCR)技术检测骨骼肌组织FoxO1及PPARγmRNA表达,Western印迹检测骨骼肌组织中FoxO1蛋白表达。结果DM组FBG、TG、TC、LDL-C、Ins和HOMA-IR水平均较NC组及HFD组显著升高(P<0.05),HFD组小鼠Ins、TC、LDL-C和HOMA-IR较NC组显著升高(P<0.05);DM组PPARγmRNA较NC组及HFD组均显著降低(P<0.01),HFD组PPARγmRNA较NC组显著降低(P<0.05);DM组FoxO1 mRNA及蛋白表达较NC组及HFD组均明显增高(P<0.05)。DM组骨骼肌FoxO1 mRNA表达与FBG(r=0.910,P<0.01)、Ins(r=0.864,P<0.01)、TG(r=0.897,P<0.01)和HOMA-IR(r=0.944,P<0.01)呈正相关,与PPARγmRNA呈负相关(r=-0.719,P<0.01)。结论FoxO1的表达与糖血脂代谢密切相关。Objective To observe the expression of FoxO1 and PPARγ inskeletal muscle of KKAy mice and explore the relation- ship between FoxO1 and glycolipid metabolism. Methods KKAy mice with the high-fat diet were chosen into the diabetic model group （DM） （n= 8） ,C57BL/6J mice were randomly divided into control group （NC） （n = 8 ）and the high-fat diet group （HFD） （n = 8）. The blood samples were used for measuring fasting blood glucose （FBG）, serum insulin （Ins）, triglyceride （TG）, total cholesterol （TC）, high density lipoprotein-C（HDL-C） and low density lipoprotein-C （LDL-C）. 16 weeks later, insulin resistance was assessed by HOMA-IR. The FoxO1 and PPARγ/mRNA expression levels in skeletal muscle were detected by real time quantitative（RT-PCR）. The FoxO1 protein expression was detected by Western bolt method. Results Levels of FBG, TG, TC, LDL-C, Ins and HOMA-IR were significantly higher in DM group than those in control group and high-fat diet group. Levels of Ins, TC, LDL-C and HOMA-IR were significantly higher in the high-fat diet group than those in control group. The mRNA expression of PPARγ in skeletal muscle of DM group（0.56±0.05） was reduced when compared with control group （1.90±0.13 ）and the high-fat diet group （0.91 ±0.06） （P〈0.01 ）, the PPARγ mRNA expression in the high-fat group was lower than that of the control group（ P〈0. 05 ）. The mRNA level of FoxO1 in skeletal muscle of DM group（48.47±2.46）was higher than that of control group（ 18.26±2.49 ）and the high-fat diet group（20.00±2.06）（ P〈0.05 ）. The protein expression of FoxO1 in skeletal muscle of DM group （4.73 ± 079 ） was higher than that of control group （0..91 ±0. 17 ）and the high-fat diet group （1.26 ±0. 16 ） （P〈0.05）. Moreover, the mRNA expression of FoxO1 in skeletal muscle was positively correlated with FBG（ r = 0. 910, P〈0. 01 ）, Ins（ r = 0. 864, P〈0.01 ）, TG （ r = 0. 897, P〈0. 01 ） and HOMA-IR （ r = 0. 944,

关 键 词：叉头状因子O1 糖尿病 过氧化物酶体增殖物激活受体 

分 类 号：R587[医药卫生—内分泌]