线叶菊抗耐药性细菌感染有效部位的抗菌活性谱效关系  

Spectrum-effect Relationships of Antibacterial Activity for Effective Parts from Filifolium sibiricum

作　　者：吕邵娃[1] 杨阳[1] 赵爽[1] 孙爽[1] 郭玉岩[1] 杨大宇[1] 李国玉[2] 王秋红 刘玉婕[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究教育部重点实验室,黑龙江省中药天然药物药效物质基础研究重点实验室,哈尔滨150040 [2]哈尔滨商业大学药学院,哈尔滨150076 [3]广东药科大学药学院,广州510006

出　　处：《中国实验方剂学杂志》2017年第24期84-89,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然基金面上项目(81373929);国家“重大新药创制”科技重大专项(2009x09103-358);黑龙江中医药大学创新人才基金项目(2012);省自然科学基金项目(H2016060)

摘　　要：目的:建立线叶菊指纹图谱,阐明共有峰所代表的化学成分对抗菌药效贡献的大小,揭示其药效物质基础。方法:采用HPLC建立线叶菊的指纹图谱,流动相乙腈-PBS(0.1 mol·L^(-1)磷酸二氢钠与2%冰乙酸1∶1混合)梯度洗脱,检测波长360 nm。通过线叶菊不同批次样品对金黄色葡萄球菌、大肠埃希菌、白色葡萄球菌、副乙型伤寒杆菌和耐药金黄色葡萄球菌的抑制作用来反映其抗菌活性,利用多元线性回归与灰色关联分析方法建立谱效关系。结果:多元线性回归法可知对耐药性金黄色葡萄球菌、大肠埃希菌、金黄色葡萄球菌、白色葡萄球菌和副乙型伤寒杆菌有作用的峰共有9个。灰关联度分析得出异鼠李素3-O-芸香糖苷、异荭草素、异杜荆苷与耐药金黄色葡萄球菌相关;异鼠李素3-O-β-D-葡萄糖苷、东莨菪内酯、杨梅素、木犀草苷、圣次草苷、异槲皮苷与大肠埃希菌和金黄色葡萄球菌相关;木犀草素和槲皮素3-O-芸香糖基-7-O-葡萄糖苷与白色葡萄球菌相关;圣次草苷、芒果苷、杨梅素、芦丁、东莨菪内酯、异鼠李素3-O-β-D-葡萄糖苷与副乙型伤寒杆菌相关,上述抑菌关联度均达到了0.8以上。结论:所建立的HPLC指纹图谱方法简便、重复性良好。Objective： To establish HPLC fingerprints and spectrum-effect relationships of different batches of Filifolium sibiricum,clarify the contribution of the chemical constituents represented by the common peaks to the antimicrobial efficacy,and reveal the pharmacological basis. Method： Fingerprint was established by HPLC method with acetonitrile-PBS（ 0. 1 mol·L^-1 sodium dihydrogen phosphate and 2% acetic acid mixed 1 ∶ 1）as the mobile phase for gradient elution and the detection wavelength was 360 nm. The inhibitory effects of different samples of F. sibiricum on Staphylococcus aureus, Escherichia coli, methicillin resistant S. aureus, Bacillus paratyphoid and S. albus were studied to reflect their antibacterial activity. Spectrum-effect relationship was revealed by multivariate linear regression and grey relational analysis method. Result： The result of multiple linear regression analysis showed that five antibacterial group experiments（ S. aureus,E. coli,methicillin resistant S. aureus,B. paratyphoid and S. albus） were developed respectively and nine peaks per group were correlated with antibacterial activities. The results of gray correlation analysis indicated that isorhamnetin-3-O-rutinoside,isoorientin and isovitexin were correlated with methicillin resistant S. aureus; E. coli and S. aureus were correlated with isorhamnetin-3-O-β-D-glucoside,scopoletin,myricetin,galuteolin,eriocitrin and isoquercitrin; S. albus was correlated with luteolin and quercetin 3-O-rutinoside-7-O-glucoside; and B. paratyphoid was correlated with eriocitrin,mangiferin,myricetin,rutin,scopoletin and isorhamnetin-3-O-β-D-glucoside. The above inhibitory correlation degrees were all more than 0. 8. Conclusion： The established HPLC fingerprint method was simple and reproducible.

关 键 词：线叶菊 细菌耐药性 指纹图谱 抗菌 谱效关系 

分 类 号：R284.1[医药卫生—中药学]