参芪解郁方对产后抑郁大鼠脑前额叶皮层,海马区5-HT,NE及其受体的干预机制  

Intervention Mechanism of Shenqi Jieyu Formula on 5-HT,NE and Their Receptors in Prefrontal Cortex and Hippocampus in Rats with Postpartum Depression

作　　者：杨歆科 赵瑞珍 李小黎 朱明瑾 吕凯 唐启盛 

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《中国实验方剂学杂志》2017年第24期112-120,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部国际科技合作项目(2010DFA30950);教育部博士点基金优先发展领域项目(20130013130002);北京中医药大学创新团队项目(2011-CXTD-26);北京中医药大学自主选题项目(2015-JYB-JSMS140)

摘　　要：目的:从脑前额叶皮层,海马5-羟色胺(5-HT),去甲肾上腺素(NE)及其受体变化角度,探讨具有健脾养心作用参芪解郁方对产后抑郁症(PPD)的干预机制。方法:选用雌性SD大鼠,2~3月龄,随机分为正常、假手术、模型、中药、西药组,每组分设1,2,4,6周观察时点。采用去势后连续雌孕激素注射并突然停撤方法制备PPD模型,并给予药物干预。规定时点取材,选脑前额叶皮层、海马区域,采用高效液相法测定5-HT,NE含量,免疫组化法检测5-羟色胺受体(5-HT_(1A)R),去甲肾上腺素受体(α_(2A)R)蛋白表达。结果:(1)模型组各时点前额叶皮层、海马5-HT,NE含量均较正常、假手术组显著减少(P<0.01)。与模型组2,4,6周比较,中药组、西药组前额叶皮层、海马5-HT,NE含量增加(P<0.05,P<0.01)。(2)与正常、假手术组各时点比较,模型组前额叶皮层、海马5-HT_(1A)R蛋白表达显著减少(P<0.01),α_(2A)R蛋白表达显著增强(P<0.01);与模型组2,4,6周比较,中药组前额叶皮层及海马5-HT_(1A)R蛋白表达增加(P<0.01);中药组2周及中、西药组4,6周前额叶皮层α_(2A)R蛋白表达减少,中药组4,6周,西药组2,4,6周海马α_(2A)R蛋白表达减少(P<0.05,P<0.01)。结论:参芪解郁方可明显提升PPD大鼠脑内5-HT,NE含量,并能对其受体表达的异常状态进行有效调整,对上述神经递质及其受体的平衡调节可能是该方发挥抗抑郁效应的重要途径之一。Objective： To use Shenqi Jieyu formula with the power of tonifying heart and spleen to treat postpartum depression（ PPD） model animals,and observe the changes in relevant prefrontal cortex,hippocampus5-serotonin（ 5-HT） and norepinephrine（ NE） and their receptors,in order to explore the intervening mechanism of traditional Chinese medicine（ TCM） in in tonifying heart and spleen. Method： Female SD rats,2 to 3 months old,were randomly divided into normal（ N） group,sham operation（ SO） group,model（ M） group,TCM group,and western medicine（ WM） group. All rats were observed respectively at 1,2,4,6 weeks after preparation. The PPD model was established through sudden withdrawal of hormones after continuous injection with estrogen and progesterone. Then,the rats were given pharmacological intervention. The rats＇ materials were collected at the prescribed time points. Their behaviors were tested at each time points. Then the contents of 5-HT,NE were detected by HPLC,and the expressions of 5-HT（1A）R,α（2A）R were detected by IHC in prefrontal cortex and hippocampus. Result： Compared with N and SO,contents of 5-HT and NE in prefrontal cortex and hippocampus in M at each time point were lower（ P〈0. 01）. Compared with M,contents of 5-HT,NE in prefrontal cortex in TCM,WM at each time point were all increased,except for at 2,4,6 weeks in TCM（ P〈0. 05,P〈0. 01）,with the same outputs in hippocampus in TCM and WM at each time point. Compared with M,levels of 5-HT（1A）R in prefrontal cortex and hippocampus in N and SO at each time point were enhanced（ P〈0. 01）,while levels of α（2A）R in above places and at the time points were attenuated（ P〈0. 01）. Expressions of 5-HT（1A）R in prefrontal cortex in TCM at 2,4,6 weeks and in hippocampus at each time point were all higher than M（ P〈0. 01）. Compared with M,expressions of α（2A）R in prefrontal cortex in TCM at 2,4,6 weeks and WM at 4,6 weeks,and in hippocampus in TCM at 4,6 weeks and WM 2,4,6 week

关 键 词：产后抑郁 前额叶皮层 海马 单胺递质及其受体 参芪解郁方 

分 类 号：R285.5[医药卫生—中药学]