人博卡病毒1型主要抗原NS1、NP1及VP1/2的抗体制备及应用  

Preparation and Application of Antibodies Against Major Antigens NS1,NP1,and VP1/2 of Human Bocavirus 1

作　　者：刘文宽[1] 游爱萍 许多[1] 邱淑燕[1] 周志超[1] 李炽 周荣[1] 

机构地区：[1]呼吸疾病国家重点实验室广州医科大学附属第一医院广州医科大学,广东广州510230

出　　处：《化学与生物工程》2017年第12期4-9,共6页Chemistry & Bioengineering

基　　金：国家自然科学基金(青年基金)项目(31500143)

摘　　要：人博卡病毒1型(human bocavirus 1,HBoV1)是全球流行的重要呼吸道病毒,其研究尚处于初期阶段。通过原核表达的方式对HBoV1主要抗原NS1、NP1、VP1/2进行蛋白表达,通过纯化、免疫小鼠成功获得3种蛋白的抗体血清,通过ELISA评价了抗体对相应蛋白抗原及临床HBoV1阳性样品的效价,并将所制备的抗体成功用于HBoV1广州株GU338055反向遗传系统的NS1、NP1、VP1/2的免疫荧光研究。结果表明,所制备抗体在1∶50稀释度时A_(450)值对阴性对照约有4倍提升,为HBoV1后续深入研究提供了重要工具,具有重要意义。Human bocavirus 1（HBoV1）is an important respiratory virus in the world,and its research is still at an early stage.We expressed the major antigens NS1,NP1,and VP1/2 of HBoV1 by prokaryotic expression,obtained successfully the antibody serums of three proteins by purification and immunization of the mouse,and evaluated the titers of antibodies by ELISA for the corresponding protein antigens and clinical HBoV1 positive sample.Meanwhile,the prepared antibodies were successfully applied in the immunofluorescence study of NS1,NP1,and VP1/2 of HBoV1 Guangzhou strain GU338055 reverse genetic system.The results indicated that,the A450 values of the antibodies prepared at 1∶50 dilution were about 4 times higher than that of the negative control,which provided an important tool for the further study of HBoV1,and would be of important significance.

关 键 词：人博卡病毒1型 NS1 NP1 VP1/2 抗体 免疫荧光 

分 类 号：R373[医药卫生—病原生物学] Q78[医药卫生—基础医学]