两株水貂阿留申全基因重组核酸疫苗的免疫效果初步评估  

Preliminary Immune Effect Evaluation of Two ADV Recombinant DNA Vaccines

作　　者：孙利杰[1] 刘东旭[2] 李健明[1] 冷雪[1] 时坤[1] 李东[1] 杜锐[3] 

机构地区：[1]吉林农业大学动物科学与技术学院,长春130118 [2]吉林农业科技学院,吉林吉林132000 [3]吉林农业大学研究生院,长春130118

出　　处：《中国兽药杂志》2017年第12期1-6,共6页Chinese Journal of Veterinary Drug

基　　金：国家自然基金(31372436);吉林省科技厅科技支撑计划(20160209006YY)

摘　　要：为研究实验室构建的两株水貂阿留申重组核酸疫苗(pcDNA3.1-ADV-428和pcDNA3.1-ADV-428-487)对水貂的免疫原性,将其经肌肉注射接种到水貂体内进行免疫,免疫完成后对水貂进行攻毒,然后每隔两周对水貂进行指尖采血。应用流式细胞术检测全血中的CD8+淋巴细胞亚群,用间接ELISA法检测血清中的抗体水平,用血清蛋白电泳检测血清中γ球蛋白占总蛋白的百分比以及应用聚乙二醇沉淀比浊法检测水貂血清中抗原抗体复合物的含量,以对两株核酸疫苗的免疫效果进行初步评估。试验结果表明,pcDNA3.1-ADV-428和pcDNA3.1-ADV-428-487的各项数据都优于对照组,且pcDNA3.1-ADV-428-487的保护效果优于pcDNA3.1-ADV-428,两种核酸疫苗都展现了良好的疫苗潜质。In this study, recombinant DNA two nucleic acid vaccines constructed by intramuscular inoculation tomink body immune（pcDNA3.1-ADV-428 and pcDNA3.1-ADV-428-487）. Mink were challenged experimentwhen after the completion of immunization, then every two weeks for blood collection of mink fingertips. The wholeblood CD8＋lymphocyte subsets detection by flow cytometry; detection of antibody levels in serum by indirectELISA; serum protein electrophoresis was used to detect the γ globulin percentage of total protein in serum andthe application of polyethylene glycol precipitation determination of antigen antibody complexes in serum of mink,the immunogenicity of two nucleic acid vaccines was evaluated preliminarily. The experimental results showed thatthe data of pcDNA3.1-ADV-428 and pcDNA3.1-ADV-428-487 were better than those of the control group, theprotective effect of pcDNA3.1-ADV-428-487 was better than that of pcDNA3.1-ADV-428, two nucleic acidvaccines had shown good vaccine potential.

关 键 词：水貂 阿留申病 核酸疫苗 免疫 抗原抗体复合物 Γ球蛋白 淋巴细胞 

分 类 号：S859.797[农业科学—临床兽医学]