高效降解多菌灵芽孢杆菌菌株D-A-2的筛选及鉴定  

Screening and Identification of Efficient Carbendazim-degrading Bacillus Strain D-A-2

作　　者：马晨 王全[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《安徽农业科学》2017年第36期135-136,220,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]筛选能高效降解多菌灵的菌株。[方法]对土壤中能高效降解多菌灵菌株D-A-2进行分离筛选及鉴定。[结果]采用管碟法从土壤中初筛得到24株具有活性的菌株,光谱法复筛得到6株降解能力较强的菌株,并对D-A-2菌株进行形态鉴定、生理生化特性鉴定和16S r DNA全序列分析。[结论]D-A-2菌株降解能力最强,初步鉴定为枯草芽孢杆菌。[ Objective ] To screen the bacterial strains that can effectively degrade carbendazim. [ Method ] Carbendazim-degrading bacillus strainD-A-2 in the soil was isolated and identified. [Result] 24 strains with activity were obtained from the soil by cylinder-plate method from the soil,6 strains with strong degrading ability were obtained by spectroscopic method. Morphological identification, phterization and sequence analysis of 16S rDNA analysis were caried out for strain D-A-2. [ Conclusion] D-ability, which was initially characterized as Bacillus subtilis.

关 键 词：多菌灵 降解细菌 分离 鉴定 枯草芽孢杆菌 

分 类 号：S482.3[农业科学—农药学]