高血压前期血浆代谢组学特征分析  

Plasma metabonomics characteristics in prehypertension

作　　者：游志刚[1] 黄琳[1] 姜醒华[1] 

机构地区：[1]南昌大学第二附属医院心血管内科,江西南昌331728

出　　处：《中国现代医学杂志》2017年第30期93-97,共5页China Journal of Modern Medicine

摘　　要：目的探讨高血压前期相关的血浆代谢变化。方法选取53例高血压前期受试者,所有受试者血压3年内反复测量均在高血压前期范围。将该组受试者与年龄和性别匹配的53例血压正常受试者的血浆代谢谱进行比较。通过超高效液相色谱线性离子阱/静电场轨道阱高分辨质谱(UHPLC-LTQ-Orbitrap MS)分析法对代谢谱进行分析。结果调整体重指数(BMI)、腰臀比(WHR)、吸烟、酗酒、血脂谱、葡萄糖和胰岛素后,高血压前期组受试者表现出更高水平的溶血卵磷脂(lyso PC),其中包含C14∶0、C16∶1、C16∶0、C18∶2、C18∶1、C18∶0、C20∶5、C20∶4、C20∶3及C22∶6,更高的脂蛋白相关磷脂酶A2(Lp-PLA2)活性、氧化型低密度脂蛋白(ox-LDL)、白介素6(IL-6)、尿8-异前列腺素F2α,以及更高臂踝脉搏波传导速度(ba PWV)。Lyso PC(16∶0)是对照组和高血压前期组的血浆代谢物比较,差异有统计学意义(P<0.05);Lyso PC与收缩压(SBP)和舒张压(DBP)均呈正相关。调整混杂变量后,高血压前期组受试者Lyso PC(16∶0)水平与ox-LDL、Lp-PLA2活性、8-异前列腺素F2α、ba-PWV和IL-6呈正相关。结论与高血压前期相关的Lyso PCs、Lp-PLA2活性、ox-LDL、尿8-异前列腺素F2α、IL-6和ba-PWV升高,表明LDL氧化升高过程中,Lp-PLA2催化PC水解,并导致氧化应激升高,从而促进炎症发展和增强动脉僵硬度。Objective To investigate the alterations of plasma metabolism in prehypertension. Methods Agroup of 53 individuals were within the range of prehypertension during repeated measurements ina 3-yearperiod. This group was compared with control group of 53 normotensive subjects who were matched for ageand gender. Metabolomic profiles were analyzed with UPLC -LTQ-Orbitrap mass spectrometry. Results Theprehypertensive group showed higher levels of lysophosphatidylcholines （lysoPCs） containing C14 ： 0, C16 ： 1,C16 ： 0, C18 ： 2, C18 ： 1, C18 ： 0, C20 ： 5, C20 ： 4, C20 ： 3, and C22 ： 6, higher activity of circulating Lp-PLA2, oxidized LDL （ox-LDL）, interleukin 6 （IL-6）, urinary 8-epi-PGF2α, and higher brachial-ankle pulse wavevelocity （baP-WV）, by adjusting for factors including BMI, WHR, smoking, alcohol consumption, serum lipidprofiles, glucose, and insulin. LysoPC （16 ： 0） was a plasma metabolite for evaluating the difference betweenthe control and the prehypertensive groups and it showed a significant statistic difference （P〈 0.05） andindependent association with DBP and SBP. The level of lysoPC （16 ： 0） in the prehypertensive group waspositively correlated with ox-LDL, Lp-PLA 2 activity, 8-epi-PGF2α, ba-PWV, and IL-6 by adjusting forconfounding variables. Conclusions Prehypertension-associated increases in lysoPCs, Lp-PLA 2 activity, ox-LDL, urinary 8-epi-PGF2α, IL-6, and ba-PWV indicate increasing oxidative stress by Lp-PLA2-catalyzedhydrolysis of PC in increasing LDL oxidation process, which enhances proinflammation and arterial stiffness.

关 键 词：高血压前期 代谢组学 溶原性卵磷脂 

分 类 号：R544.1[医药卫生—心血管疾病]