2,3-丁二醇诱导下匍匐翦股颖叶片细胞结构变化及抗病相关性分析  

The correlation between cell microstructure induced by 2,3-butanediol and fungal resistance of creeping bentgrass

作　　者：刘兴菊 马源 马晖玲[1,2,3] 张咏梅 余倩倩[5] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]草业生态系统教育部重点实验室,甘肃兰州730070 [3]中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [4]甘肃农业大学研究测试中心,甘肃兰州730070 [5]华南农业大学园艺学院,广东广州510642

出　　处：《草业学报》2017年第12期170-178,共9页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(31360583)资助

摘　　要：由立枯丝核菌(Rhizoctonia solani)引起的褐斑病是匍匐翦股颖草坪最常见的病害之一,2,3-BD(2,3-butanediol)可诱导植物产生抗病反应,提高植物的抗病性。本试验采用250μmol/L 2,3-BD诱导匍匐翦股颖对褐斑病的抗性,以石蜡切片方法通过显微观察,测定接种立枯丝核菌后匍匐翦股颖叶片细胞显微结构的变化。结果表明,接种病原菌后匍匐翦股颖病情指数第15天达到70.45%,2,3-BD诱导后病情指数显著降低至21.63%。与未诱导接菌处理相比,诱导后匍匐翦股颖叶片细胞结构排列紧密,气腔变小,有较多乳突形成,上、下表皮细胞显著增大,第7天差异最显著,分别是其1.19和1.26倍;叶肉细胞大小于第15天差异最为显著,是其1.30倍;叶绿体数量明显增加,第1、7、15天分别是未诱导接菌处理的1.25、1.20、1.24倍;维管束和导管大小在各时间段均变化不明显,但木质化程度较高,皮层组织加厚。综上所述,250μmol/L的2,3-BD能诱导匍匐翦股颖对褐斑病的抗性,并使细胞结构发生变化,其中叶片上、下表皮细胞、叶肉细胞大小和叶绿体数目与抗病性呈正相关性。The brown spot caused by Rhizoctonia solani is one of the most common diseases of creeping bentgrass（Agrostis stolonifera） lawns. Application of 2,3-butanediol（2,3-BD） can induce plant disease response and improve disease resistance. In the present study,creeping bentgrass was inoculated with R. solani after treatment with 2,3-BD（250 μmol/L） and then the cell microstructure of leaves was observed using a paraffin sectioning method. It was found that the disease index of creeping bentgrass without 2,3-BD reached 70. 45% after 15 days from inoculation. However,the disease index of creeping bentgrass treated with 2,3-BD was significantly lower,at 21. 63%（ P〈0. 05）. Compared to leaves of pathogen-infected plants untreated with 2,3-BD,creeping bentgrass leaves with both pathogen inoculation and 2,3-BD treatment had a closely arranged cell structure,smaller cavities,more papillae,and increased upper and lower epidermal cell size. The size increase of upper and lower epidermal cells of 2,3-BD induced plants,compared to uninduced plants,was a factor of 1. 19 and 1. 26 times,respectively,and was most significant on day 7 after inoculation. The mesophyll cell size increase was the most significant on the 15 th day（ × 1. 30）. The chloroplast density 1,7,and 15 days after inoculation was,respectively,1. 25,1. 20,and 1. 24 times that of uninduced plants（ P〈0. 05）. The size of vascular bundles and ducts did not change significantly at any time,however,the degree of lignification was higher and the cortical tissue became thicker in induced plants. In conclusion,2,3-BD can induce resistance of creeping bentgrass to brown spot disease through change in the cell structure. There was a positive correlation between the upper and lower epidermal cell size,the mesophyll cell size,and the number of chloroplasts per cell in creeping bentgrass leaves,and the disease resistance.

关 键 词：2 3-BD 匍匐翦股颖 石蜡切片 细胞结构 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]