反复种植失败患者CD8^+T细胞数量及功能的研究  

Study on quantity and function of CD8^+T cells in patients with repeated implantation failure

作　　者：徐士儒 李玉叶 连若纯 余姝毅 许健 陈聪 曾勇 

机构地区：[1]广东省深圳市中山泌尿外科医院生殖中心,深圳市围着床期生殖免疫重点实验室,深圳中山生殖与遗传研究所,518045

出　　处：《实用医学杂志》2017年第23期3885-3890,共6页The Journal of Practical Medicine

基　　金：国家自然基金项目(编号:8160060585);中华医学会临床医学科研专项资金-生殖医学青年医师研究与发展项目(编号:16020200636);深圳市科技计划基础研究项目(编号:JCYJ20150402165325176)

摘　　要：目的探讨反复种植失败(RIF)患者中周围血及子宫内膜CD8^+T细胞的数量及功能变化。方法在黄体中期,对37例来本中心就诊的RIF患者周围血及子宫内膜进行取样。流式及免疫组化分析CD8^+T细胞的数量,流式检测周围血CD8^+T细胞表达穿孔素及颗粒酶B的情况,免疫荧光检测子宫内膜CD8^+T细胞穿孔素及颗粒酶B的表达情况。结果与正常对照组相比较,RIF患者周围血CD8^+T细胞数量(37.22%vs.37.15%,P>0.05)无明显差异。而子宫内膜CD8^+T细胞的数量(1.99%vs.3.77%,P<0.001)显著高于正常对照组。周围血和子宫内膜CD8^+T细胞表达的穿孔素和颗粒酶B在正常对照组和RIF组之间均无统计学变化。结论与正常对照组相比较,RIF患者子宫内膜CD8^+T细胞的数量显著增多,但周围血和子宫内膜CD8^+T细胞的杀伤功能无显著差异,说明CD8^+T细胞的杀伤功能可能不是导致RIF发生的关键因素,但具体的分子机制还需进一步研究。Objective To investigate the quantity and function of CDS^T cells in peripheral blood of pa- tients with repeated implantation failure （RIF）. Methods 37 patients with RIF and 19 healthy controls were en- rolled in this study. The peripheral blood and endometrium were collected during the mid-luteal＂ phase. The percent- age of peripheral CD8＋T subsets and the levels of perforin and granzyme B in peripheral CD8＋T ceils were deter- mined by flow cytometry assay. The percentage of endometrial CD8＋T cells was detected by IHC, the production of perforin and granzyme B in endometrial CD8＋T ceils was detected by IF. Results Compared with the control group, the percentage of peripheral CD8＋T cells in patients with RIF was not significantly changed （37.22% vs. 37.15%, P 〉 0.05）. However, the porportion of endometrial CD8＋T cells in the RIF group was higher than that in the control group （ 1.99% vs. 3.77%, P 〈 0.001 ）. The levels of perforin and granzyme B in peripheral blood and en- dometrial CD8＋T cells in patients with RIF were similar with those in the control group. Conclusions Compared to the control group, the percentage of endometrial CD8＋ T was markedly upregulated in patients with RIF. However, the production of perforin and granzyme B were similar between the control group and the RIF group.

关 键 词：反复种植失败 CD8+T细胞 穿孔素 颗粒酶 

分 类 号：R714.8[医药卫生—妇产科学]