激动素对AlCl_3和D-半乳糖联合诱导的AD小鼠模型大脑病理变化和Aβ表达的影响  

Effects of Kinetin on Pathological Lesions and Aβ Expression in Brain of AD Mouse Model Induced by AlCl_3 and D-galactose Combination Treatment

作　　者：刘丹[1] 魏云鹏[1] 欧阳五庆[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100

出　　处：《动物医学进展》2017年第12期68-72,共5页Progress In Veterinary Medicine

基　　金：陕西省重大科技创新专项基金(K332020916)

摘　　要：旨在研究激动素对AD小鼠模型的神经保护作用。首先用AlCl_3和D-半乳糖联合处理制备AD小鼠模型,然后用不同浓度的激动素进行干预处理,再通过HE染色对大脑海马CA3区进行病理学观察,并对大脑皮层和海马部位β淀粉样蛋白(βamyloid protein,Aβ)进行免疫组化染色。结果表明,AlCl_3和D-半乳糖处理过的小鼠其海马CA3区锥体细胞发生明显的病理变化,而激动素的干预减轻了这一病变,且表现出剂量依赖性。同时,AlCl_3和D-半乳糖处理过的小鼠与空白组小鼠相比,大脑皮层和海马区Aβ1-42分布均显著增加,但激动素干预能够使Aβ1-42的表达和分布明显减少,且显示出剂量依赖性的特点。这些结果说明激动素对AD小鼠模型具有一定的正向干预作用,表明激动素可能具有抗AD的潜力。The aim of this study was to investigate the neuroprotective effects of kinetin on AD mouse models.The AD mouse models were prepared by AlCl3 and D-galactose,and at the same time were treated with different concentrations of kinetin.The hippocampal CA3 region was observed by HE staining.Immunohistochemical staining of β amyloid protein （A;） in the cortex and hippocampus was also performed.The results showed that the pyramidal cells in the hippocampal CA3 region of the mice treated with AlCl3 and Dgalactose had a significant pathological change, but the intervention of kinetin reduced the lesion. At the same time,the distribution of Aβ1-42 in cortex and hippocampus was significantly increased compared with the blank group,but that in kinetin intervention group was significantly decreased.All the results showd ki- netin dose-dependent characteristics. These results indicated that kinetin has some positive effects on AD mouse models,and may has the potential to fight AD.

关 键 词：激动素 三氯化铝 D-半乳糖 病理变化 AΒ 

分 类 号：S852.2[农业科学—基础兽医学]