H7N9禽流感患者外周血T淋巴细胞亚群及细胞因子检测  

T lymphocyte subsets and Cytokines of patients infected with influenza A(H7N9) virus

作　　者：何纲[1] 陈晓华[1] 伍金华[1] 李秀娟[1] 甄沛林[1] 丁佩佩[1] 梁少群[1] 

机构地区：[1]江门市中心医院,广东江门529030

出　　处：《热带医学杂志》2017年第11期1454-1456,1460,共4页Journal of Tropical Medicine

基　　金：江门市科技局科研基金项目(2014018)

摘　　要：目的探讨H7N9禽流感患者外周血细胞因子变化。方法收集H7N9禽流感患者外周血,用流式细胞仪检测T淋巴细胞亚群百分率,ELISA法检测干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-4(IL-4)及白细胞介素-10(IL-10)细胞因子水平,并与正常对照组进行比较分析。结果 10例患者外周血CD4+、CD8+T淋巴细胞百分率为25.26(15.83~33.34)、15.01(9.23~20.33),较对照组明显减少,差异有统计学意义(P<0.05)。Th2型T淋巴细胞为2.31(0.40~4.59),较对照组明显增高,差异有统计学意义(P<0.05)。外周血细胞因子IFN-γ、TNF-α、IL-10水平分别为35.51(18.54~46.23)ng/L、40.65(23.12~65.87)ng/L和5.34(3.25~6.32)ng/L,均明显高于对照组,差异有统计学意义(P<0.05)。而IL-4水平为6.11(2.51~8.14)ng/L,与对照组比较差异无统计学意义(P>0.05)。结论 H7N9禽流感患者存在T淋巴细胞亚群及Th1、Th2免疫失调,多种细胞因子上调,参与机体免疫损伤。Objective To explore the T lymphocyte subsets and cytokines changes in peripheral blood of patients infected with influenza A （H7N9） virus. Methods Patients were divided into H7N9 group and control group. Percentages of T lymphocyte subsets CD4＋,CD8＋,Th1 and Th2 in peripheral blood were detected by flow cytometry. Interferon-γ（IFN-γ）, tumor necrosis factor-α（TNF-α）,IL-4 and IL-10 levels in peripheral blood were detected by ELISA. Results The percentages of T lymphocyte subsets CD4＋,CD8＋25.26（15.83~33.34）,15.01（9.23~20.33）were significantly lower than those of the control group（P ＜ 0.05）. The percentages of T lymphocyte subsets Th22.31（0.40~4.59）was significantly higher than those of the control group（P＜0.05）. IFN-γ,TNF-α,IL-1035.51（18.54~46.23）ng/L,40.65（23.12~65.87） ng/L,5.34（3.25~6.32）ng/L in peripheral blood of H7N9 group were significantly higher than those of the control group （P ＜ 0.05）. There were no significant difference in IL-4 between the 2 groups（P ＞ 0.05）. Conclusion Imbalance of T lymphocyte subsets ,Th1/Th2 and up-regulation of several cytokines and cytokine storm were found in patients with severe influenza A（H7N9）infection,and could lead to impair of the immune system.

关 键 词：禽流感H7N9亚型病毒 T淋巴细胞亚群 细胞因子 

分 类 号：R373.13[医药卫生—病原生物学]