电针对偏头痛大鼠中脑导水管周围灰质5-羟色胺7受体和血浆降钙素基因相关肽表达的影响  

Effect of Electroacupuncture on Expression of 5-HT_7 Receptor in Periaqueductal Gray and Plasma Calcitonin Gene-related Peptide in Migraine Rats

作　　者：裴培[1] 陈怀珍[1] 王艳昕[1] 杨文明[1] 刘璐[2] 王麟鹏[2] 

机构地区：[1]安徽中医药大学第一附属医院脑病中心,合肥230031 [2]首都医科大学附属北京中医医院针灸中心,北京100010

出　　处：《针刺研究》2017年第6期510-513,556,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划"973"中医专项(No.2014CB 543203);国家自然科学基金资助项目(No.81603683)

摘　　要：目的:探讨电针是否能通过调控中脑导水管周围灰质(PAG)5-羟色胺7(5-HT7)受体和外周血降钙素基因相关肽(CGRP)介导抗偏头痛。方法:雄性SD大鼠随机分为对照组、模型组和电针组,每组14只。反复电刺激大鼠上矢状窦区硬脑膜诱发神经源性偏头痛模型。对照组仅予颅内电极安置,电针组于硬脑膜电刺激后电针"风池"穴,每日1次,治疗6次。采用免疫荧光标记法和Western blot法检测PAG区5-HT7受体的表达,放射免疫法检测外周血CGRP含量。结果:与对照组比较,反复硬脑膜电刺激造模后PAG区5-HT7受体阳性神经元数目和蛋白相对表达量及外周血CGRP含量显著增高(均P<0.001);与模型组比较,电针组5-HT7阳性神经元数目和蛋白相对表达量显著降低(P<0.01),外周血CGRP含量降低(P<0.05)。结论:电针"风池"穴可下调偏头痛大鼠PAG区5-HT7受体表达,降低其外周血CGRP含量。Objective To explore the analgesic effect of electroaoupuncture（EA）by modulating 5-hydroxytryptamine 7 （5-HTT） receptor in periaqueductal gray （PAG） and plasma calcitonin gene-related peptide （CGRP）. Methods Forty-two male Sprague Dawley （SD） rats were randomly divided into control,model and EA groups, 14 rat in each one. The neurogenic migraine model was established by repeated electrical stimulation on sagittal sinus duramater. Intracranial electrodes were used in the control group without stimuli. The rats in the EA group received EA （0.5- 1 mA, 2 Hz/15 Hz） at ＂Fengchi＂ （GB 20） for 10 min after dural electrical stimulation, once a day for 6 days. The expression of 5-HT7 receptor in the PAG was assessed by immunofluorescence and Western blot, respectively; plasma CGRP was measured by radioimmunoassay. Results Compared with the control group, the positive neuron number and protein expression of 5-HT7 receptor in PAG and plasma CGRP increased after model establishment （all P〈0. 001）. The above mentioned indexes were reversed in the EA group compared with those in the model group （the positive neuron number and protein expression of 5-HT7 receptor, P〈0.01 ; plasma CGRP, P〈0.05）. Conclusion EA at GB 20 can down-regulate the expression of 5-HT7 receptor in the PAG and reduce the content of plasma CGRP in the rats of migraine.

关 键 词：偏头痛 电针 中脑导水管周围灰质 5-羟色胺7受体 

分 类 号：R245.97[医药卫生—针灸推拿学]