针刀干预对颈椎病兔颈后伸肌B淋巴细胞瘤-2及其相关的x基因、天冬氨酸特异性半胱氨酸蛋白酶-3 mRNA表达的影响  

Effects of Acupotomy Therapy on mRNA Expressions of Bcl-2,Bax,Caspase-3 in Posterior Cervical Extensor Muscles in Cervical Spondylosis Rabbits

作　　者：刘福水[1] 周凡媛 张义[2] 郭长青[2] 

机构地区：[1]江西中医药大学灸学院,南昌330004 [2]北京中医药大学针灸推拿学院,北京100029

出　　处：《针刺研究》2017年第6期514-517,共4页Acupuncture Research

基　　金：国家自然科学基金(No.81560792);江西省教育厅科技计划项目(重点项目;No.GJJ 150834);江西省卫生计生委中医药科研项目(重点课题;No.2016Z03)

摘　　要：目的:观察针刀干预对颈椎病兔颈后伸肌B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关的x基因(Bax)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)mRNA表达的影响,探讨针刀松解法对劳损颈肌细胞凋亡影响的可能作用机制。方法:新西兰兔随机分为空白组、模型组、电针组及针刀组,每组6只,采用长期低头位方法建立颈椎病动物模型。造模成功后针刀组选取斜方肌起点及胸锁乳突肌附着点等部位进行针刀治疗,每周治疗1次,共治疗3次;电针组电针"天柱""颈百劳""大杼"穴,每次20min,每周治疗3次,共治疗3周。实时荧光定量PCR法检测颈后伸肌Bcl-2、Bax、Caspase-3mRNA表达水平。结果:各组间颈后伸肌Bcl-2mRNA表达水平比较差异无统计学意义(P>0.05)。模型组颈后伸肌Bax mRNA表达较空白组明显升高(P<0.01),针刀组较模型组明显降低(P<0.01)。模型组颈后伸肌Bcl-2/Bax mRNA比值较空白组明显降低(P<0.01),针刀组较模型组明显升高(P<0.01),针刀组较电针组明显升高(P<0.05)。模型组颈后伸肌Caspase-3mRNA较空白组升高(P<0.05),针刀组较模型组及电针组均明显降低(P<0.05)。结论:针刀干预可调节Bax、Bcl-2/Bax Caspase-3mRNA表达,减缓颈后伸肌细胞凋亡进程,从而修复劳损颈肌,可能是针刀治疗颈椎病作用机制之一。Objective To observe the effect of acupotomy therapy on mRNA expressions of Bcl-2, Bax , Caspase-3 in posterior cervical extensor muscles in cervical spondylosis rabbits, and explore its mechanisms for apoptosis of cervical muscles. Methods New Zealand rabbits were randomly divided into normal, model, electroacupuncture （EA） and acupotomy groups, 6 in each group. The cervical spondylosis model was established by forced head-bowing for a long term. After model establishment, acupotomy was used at the trapezius muscle starting point and attachment site of sternocleidomastoid muscle, etc., once a week, total 3 times. EA was used at ＂Tianzhu＂ （BL 10）, ＂Jingbailao＂ （EX-HN 15） and ＂Dazhu＂ （BL 11） for 3 weeks, 20 min a time, 3 times a week. Bcl-2, Bax, Caspase-3 mRNA expressions in posterior cervical extensor muscles were detected by real-time PCR. Results There was no significant difference in the expression of Bcl-2 mRNA among all the groups （P〉0.05）. Compared with the normal group, Bax mRNA increased in the model group （P〈0. 01 ）, and that in the acupotomy group was lower than that in the model group （P〈0. 01 ）. The ratio of Bcl-2/Bax mRNA in the model group decreased significantly compared with that in the normal group （P〈0.01）; in comparison with the model group, the ratio in the acupotomy group increased （P〈0.01）; and that in the acupotomy group was higher than the ratio in the EA group （P〈0.05）. The Caspase-3 mRNA expression in the model group increased compared with that in the normal group （P〈0.05）, and its expression in the acupotomy group decreased compared with those in the model and EA groups （P〈0.05）. Conclusion Acupotomy therapy can regulate the mRNA expressions of Bax and Caspase-3, and retard apoptosis in posterior cervical extensor muscles, therefore the strained muscles are relieved, which may be one of its mechanisms for improving cervical spondylosis.

关 键 词：针刀 颈椎病 细胞凋亡 B淋巴细胞瘤-2基因 B淋巴细胞瘤-2相关的x基因 天冬氨酸特异性半胱氨酸蛋白酶-3基因 

分 类 号：R246.2[医药卫生—针灸推拿学]