花生组培再生及农杆菌介导遗传转化研究进展  

Tissue Culture Regeneration and Agrobacterium Mediated Transformation of Peanut: Research Progress

作　　者：苗利娟[1] 黄冰艳[1] 张新友[1] 董文召[1] 刘娟[1] 秦利[1] 张俊[1] 齐飞艳[1] 石磊[1] 

机构地区：[1]河南省农业科学院经济作物研究所/农业部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室,郑州450002

出　　处：《中国农学通报》2017年第32期15-20,共6页Chinese Agricultural Science Bulletin

基　　金：国家高技术研究与发展计划(863计划)项目(2013AA102602-6);河南省重大科技专项(16110011100);河南省农业科学院自主创新项目(2017ZC20);河南省现代农业产业技术体系项目"河南省花生产业技术体系"(S2012-5)

摘　　要：农杆菌介导法是花生遗传转化最常用的转化方法,近年来,花生农杆菌介导转化技术日渐成熟,但仍存在再生体系不完善,以及转化效率低和基因型限制等难题。为了建立高效的再生和遗传转化体系,本文归纳了近十几年来国内外花生组织培养及农杆菌介导转化方法,分析了不同方法的诱导效率和转化效果,认为花生体胚转化体系可以提高外源基因的遗传稳定性,并克服嵌合体等问题。在现有的组培再生基础上,对花生体胚诱导再生进一步优化,建立高频的花生体胚再生体系,进一步提高花生的转化效率,建立标准化、规模化、工厂化以及可重复的安全高效转化体系,是花生遗传转化的发展趋势。Agrobacterium-mediated transformation is the most common genetic transformation method for peanuts （Arachis hypogaea L）. In recent years, the technology of Agrobacterium-mediated transformation for peanut matures gradually. However, there are still barriers such as incomplete regeneration system, low transformation efficiency and genotype restriction. In order to establish an efficient regeneration and genetic transformation system for peanuts, in this paper, the tissue culture and Agrobacterium-mediated transformation of peanut at home and abroad in recent years were summarized. The induction efficiency and transformation effect were analyzed. It indicated that the embryo transformation system could improve the stability of exogenous genes and overcome the chimera problems. Based on the existing tissue culture regeneration systems, the embryo regeneration of peanut should be optimized, the high-frequency embryo regeneration system should be established, the transformation efficiency should be improved, and the safe and efficient transformation system with standardization, scale, industrialization and repeatability should be set up, which are the development trends of peanut genetic transformation.

关 键 词：花生 组织培养 再生 农杆菌介导转化 

分 类 号：S565.2[农业科学—作物学]