NaCl对旱柳生长发育及部分生理特性的影响  

Effects of NaCl on seedling growth and some physiological characteristics of Salix matsudana Koidz.

作　　者：李斌彬[1,2] 欧阳洁[1,2] 王嘉玥 吴航枫[1,2] 刘祥君[1,2] 邹金华[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2017年第6期37-42,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市自然科学基金资助项目(17JCYBJ22500)

摘　　要：为了探究旱柳对盐胁迫的生长和生理响应机制,以旱柳无性系扦插枝条为材料,用含有不同质量浓度NaCl的Hoagland营养液对其进行水培,观察旱柳枝条和根的生长情况,测量旱柳的光合与气体交换指标、抗氧化酶活性及丙二醛(MDA)含量.结果表明,盐胁迫会抑制旱柳枝条和根的生长,随着盐胁迫程度的增加,旱柳的净光合速率、气孔导度、胞间CO_2浓度和蒸腾速率等急剧下降,气孔抑制百分率和水分利用效率显著升高.低浓度盐胁迫会诱导SOD、POD和CAT活性的升高,高浓度盐胁迫则会抑制抗氧化酶活性.旱柳MDA的含量在低盐胁迫下略增加,随着盐胁迫程度的增加,MDA含量迅速升高.总之,旱柳在NaCl质量浓度为0.1~0.2 g/L的培养液中能够生长;当NaCl质量浓度达到0.4 g/L时,旱柳生长受到严重抑制.对盐胁迫下旱柳光合抑制的分析表明,气孔因素是导致旱柳叶片光合速率降低的主要原因.To explore the growth and physiological responses of Salix matsudana Koidz. exposed to salt stress, the cutting shoots were cultivated in Hoagland nutrient solutions with different mass concentrations of NaCl. The growth conditions of shoot and root were observed, and the physiological features including photosynthesis and gas exchange parameters, antioxidant enzyme activity and MDA content were measured. The results showed that the NaCl stress can inhibit the growth of shoot and root of S. matsudana. With the increase of mass concentrations of NaCl, the net photosynthetic rate, stomatal conductance, intercellular CO： concentration and transpiration rate decreased sharply, the percentage of stomatal limitation and water use efficiency increased significantly. At low mass concentrations of NaCl, the activities of SOD, POD and CAT of S. matsudana increased significantly, while at high mass concentration of NaCl, the activities of antioxidant enzyme were inhibited. The MDA content of S. matsudana increased slightly when exposed to the low NaCl stress. With the increase of mass concentrations of NaCl, the MDA content increased sharply. Overall, the shoots of S. matsudana can grow cultivated in the Hoagland solutions with the NaCl mass concentrations of 0.1-0.2 g/L. While the NaCl mass concentrations of 0.4 g/L can inhibit the growth of S. matsudana. The analysis of the mechanisms of photosynthetic inhibition when exposed to NaCl stress indicated that the decrease of photosynthetic rate of S. matsudana was mainly due to the stomatal factors.

关 键 词：旱柳 NACL胁迫 光合与气体交换参数 抗氧化酶活性 MDA含量 

分 类 号：Q945[生物学—植物学]