丹瓜方对ApoE^(-/-)糖尿病模型小鼠脑组织Caspase-3蛋白及Bcl-2、Bax mRNA表达水平的影响  

Effect of Dangua Recipe on Expression Levels of Caspase-3 Protein,Bcl-2 and Bax mRNA in Brain Tissue of ApoE^(-/-) Diabetes Model Mice

作　　者：陈依楚 李亮 衡先培 王志塔 黄苏萍 杨柳清 单丽[4] 颜群芳 庄怀山 陈玲[5] 姚淑红 张治三 

机构地区：[1]福建中医药大学附属人民医院福建省人民医院内分泌科,福州350004 [2]广州中医药大学第二临床医学院,广州510000 [3]福建中医药大学中西医结合研究院,福州350122 [4]福建中医药大学附属人民医院药学部,福州350004 [5]福建中医药大学生理学教研室,福州350122

出　　处：《中国中西医结合杂志》2017年第12期1476-1481,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173179;No.81473550;No.81403329);福建省科技社会发展重点项目(No.2012Y0037);福建省卫生厅中医药科研重点研究室基金(No.wztn 201304);陈可冀中西医结合发展基金资助项目(No.CKJ2009004);福建中医药大学重点学科专项(No.X2014037)

摘　　要：目的研究丹瓜方对糖尿病模型ApoE^(-/-)小鼠糖脂代谢、脑组织胱冬肽酶-3(Caspase-3)蛋白表达及β淋巴细胞瘤基因-2(Bcl-2)、Bax mRNA表达水平的影响。方法采用链脲佐菌素(streptozo tocin,STZ)注射法对ApoE^(-/-)小鼠制备糖尿病模型。将成模后的32只小鼠按体重分层,再按血糖水平排序,采用随机数字表分为模型组[无菌水(SW),15mL/(kg·d)]、丹瓜方组[丹瓜方(DGR),15mL/(kg·d)]、联合组[吡格列酮(PG)4.3mg/(kg·d)+DGR 15mL/(kg·d)]及吡格列酮组[PG,4.3mg/(kg·d)],每组8只。另选血糖正常的8只同龄同品系C57小鼠作为对照组。灌胃干预12周。监测空腹血糖(fasting blood glucose,FBG),测定血清TC、LDL-C水平;实时荧光定量PCR(qPCR)法检测ApoE^(-/-)小鼠脑组织Bcl-2、Bax mRNA表达,采用Western blot法测定脑组织Caspase-3蛋白表达水平。结果与对照组比较,模型组ApoE^(-/-)小鼠血清FBG、TC、LDL-C及脑组织Caspase-3表达水平升高(P<0.01);与模型组比较,丹瓜方组FBG、TC及LDL-C水平降低,Bcl-2、Bax mRNA水平升高(P<0.01,P<0.05)。丹瓜方组Caspase-3蛋白表达水平低于模型组、联合组及吡格列酮组各组(P<0.01);联合组Bcl-2/Bax比值高于模型组(P<0.05),丹瓜方组高于吡格列酮组(P<0.01)。结论丹瓜方可调节糖尿病糖脂代谢及脑组织Caspase-3蛋白及Bcl-2、Bax mRNA表达,可能对糖尿病造成的脑组织细胞凋亡有一定的防治作用。Objective To observe the effects of Dangua Recipe （DGR） on glycolipid metabo- lism, Caspase-3 protein expression, and mRNA expression levels of Bcl-2 and Bax in brain tissue of transgenic ApoE-/-model mice. Methods Diabetes model was prepared by peritoneally injecting strepto-zotocin （STZ） to ApoE-/- mice. Totally 32 successfully modeled mice were stratified by body weight, and then sorted by blood glucose level from low to high. They were randomly divided into the model group [ MOD, fed with sterile water （SW）, at the daily dose of 15 mL/kg], the DGR group （at the daily dose of 15 mL/kg）, the combination group [COM （fed with DGR at the daily dose of 15 mL/kg） and pioglitazone （at the daily dose of 4. 3 mg/kg） ], and the pioglitazone group （PG, at the daily dose of 4.3 mg/kg） according to random number ta- ble, 8 in each group. Besides, another 8 euglycemia C57 mice of the same age and strain were recruited as the control group. The intervention lasted for 12 weeks by gastrogavage. Fasting blood glucose （FBG） was mo- nitored. Levels of total cholesterol （TC） and low-density lipoprotein cholesterol （LDL-C） were detected as well. mRNA expressions of Bcl-2 and Bax in brain tissue were detected by Real-time fluorescent quantitative PCR （qPCR）. Caspase-3 protein expression level in brain tissue was detected by Western blot. Results Levels of serum FBG, TC, LDL-C, and Caspase-3 expression in brain tissue were higher in the MOD group than in the control group （P 〈0. 01 ）. Levels of FBG, TC, LDL-C were lower in the DGR group than in the MOD group, while mRNA expressions of Bcl-2 and Bax were higher in the DGR group than in the MOD group （P 〈0. 01, P 〈0. 05）. Caspase-3 expression level was lower in the DGR group than in the other groups （P 〈0. 01 ）. Bcl-2/Bax ratio was higher in the COM group than the MOO group （P 〈0.05）. Bcl-2/Bax ratio was higher in the DGR group than the PG group （P 〈0. 01 ）. Conclusions DGR could regulate glycolipid metabolism

关 键 词：丹瓜方 糖尿病 脑损害 糖脂代谢 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]