利用SSR标记划分广西骨干玉米自交系的杂种优势群  

Analysis Heterotic Groups of Elite Maize Inbred Lines in Guangxi by SSR Makers

作　　者：杜青[1] 郑加兴[1] 吕巨智[1] 文仁来[1] 曾艳华[1] 杨耀炯 王治红 张述宽[3] 唐照磊[1] 李石初[1] 程伟东[1] 

机构地区：[1]广西农业科学院玉米研究所,南宁530007 [2]南宁市正昊农业科学研究院,南宁530003 [3]广西农业科学院,南宁530007

出　　处：《玉米科学》2017年第6期21-27,共7页Journal of Maize Sciences

基　　金：国家现代农业产业技术体系南宁玉米综合试验站项目(CARS-02-73);国家现代农业产业技术体系广西创新团队建设岗位专家项目(nycytxgxcxtd-04-03);广西自然科学基金青年基金项目(2014GXNSFBA118121);广西农业科学院基本业务专项(桂农科2015YM02)

摘　　要：利用SSR标记对广西骨干玉米自交系进行遗传多样性分析和杂种优势类群划分,60对标准引物在33份供试材料中共检测出233个等位基因,平均多态性信息量为0.77,自交系间平均遗传相似系数为0.49。研究结果表明,这些自交系来源广泛,且具有较好的遗传基础。按照UPGMA方法进行聚类分析,可以将33份玉米自交系分为Ⅰ和Ⅱ两个类群,Ⅰ类群可进一步划分为4个亚群。杂种优势模式可被简化为"A×B"模式,A群包括Ⅰ-Ⅰ亚群、Ⅰ-Ⅱ亚群和Ⅰ-Ⅲ亚群自交系,B群包括Ⅰ-Ⅳ亚群和第Ⅱ类群自交系。Simple sequence repeats（SSR） primers were employed to analyze the genetic diversity and to classify the heterotic groups of elite inbred lines that be collected from Guangxi. Two hundred thirty-three alleles were de- tected by 60 markers upon 33 provided materials, the average PCI was 0.77, and the average index of genetic simi- larity was 0.49 among these inbred lines. The results indicated that the resources and genetic background of these inbreds were broad. The 33 inbreds were clustered into group I and group Ⅱ by UPGMA method. The group I was divided into 4 subgroups further. Heterotic group pattern could be simplified as pattern ＂AxB＂. Group A included inbred lines of subgroups I - Ⅰ , 1 - Ⅱ and Ⅰ -Ⅲ. Group B included inbred lines of subgroup I -Wand group .

关 键 词：玉米 骨干自交系 SSR 聚类分析 杂种优势群 

分 类 号：S513.035.1[农业科学—作物学]