聚乙烯醇-卟啉新型纳米颗粒的制备及其光热特性  

Preparation of new type polyvinyl alcohol-porphyrin nanoparticles and its photothermal properties

作　　者：李军果 王全晖[2] 史海江 宋宗昌[4] 罗焱 

机构地区：[1]解放军陆军总医院生殖中心,北京100700 [2]解放军第153中心医院普外科,郑州450042 [3]解放军第153中心医院肿瘤科,郑州450042 [4]解放军第155中心医院血液肿瘤科,河南开封475003

出　　处：《重庆医学》2017年第35期4901-4903,4907,共4页Chongqing medicine

基　　金：总后卫生部青年培育项目(14QNP030)

摘　　要：目的将一定比例的脱镁叶绿酸引入聚乙烯醇-硫辛酸聚合物中,分析其理化性质及光热特性。方法采用"一步法"合成聚乙烯醇-硫辛酸-脱镁叶绿酸(PLP)聚合物,激光粒径测定仪测定PLP纳米颗粒(PLPN)的粒径、多分散系数和ζ电位,电镜下观察形态,分光光度法和热成像仪初步探索其体外荧光和光热特性;建立卵巢癌裸鼠模型,行初步的体内实验评价。结果合成的PLP聚合物在水相中自组装形成规则的球状纳米颗粒,电镜下呈紧密的球状结构,平均粒径、多分散系数和ζ电位分别为(22.92±9.00)nm、0.117±0.056和(-25.43±0.77)mV;PLPN在磷酸盐缓冲液(PBS)中的荧光强度明显低于十二烷基硫酸钠(SDS)溶液(P<0.05),而近红外光照后温度明显高于SDS溶液(P<0.05);在荷瘤裸鼠模型中,PLPN可靶向聚集在肿瘤部位,近红外光激发后能明显提高肿瘤部位的温度,并能杀伤肿瘤细胞。结论 PLPN发射红色荧光,能靶向聚集在肿瘤部位并行活体显像,且在体内外均具有显著的光热转换特性,在体内能有效抑制肿瘤的生长。Objective To analyze the physieochemical properties and photothermal properties after introducing a certain proportion of phaeophorbide into polyvinyl alcohol-lipoic acid polymer. Methods The ＂one-step＂ method was adopted to synthesize the PVA27K-4 % LA-10% Pheide （PLP） polymer. The laser particle diameter tester was used to detect the particle size,polydispersity index and ξ potential of PLP nanoparticle （PLPN） ;the morphology was observed under transmission electron microscope （TEM） the in vitro fluorescent and photothermal properties were investigated by using spectrophotometric method and infrared thermal im- ager;the ovarian-cancer nude mice models were established and the preliminary in vivo experiment evaluation was performed. Results The synthetic PLP polymer could self-assembly form spherical PLPN in aqueous environment, which showed the tightly spherical structure under TEM. The average particle diameter, polydispersity index and ξ potential were （22. 92 i 9. 00）nm, （0. 117 ~ 0. 056） and （- 25.43±0. 77）mV respectively. The fluorescence intensity of PLPN in PBS solution was significantly lower than that in SDS solution （P〈0. 05）. However,the temperature of the PBS solution of PLPN was significantly higher than that of the SDS solution after exposure to the near infrared light （P〈0.05）. In the model of tumor-bearing nude mice,PLPN could targetaccumulated in the tumor site. The temperature in the tumor site was significantly increased after exposure to the near infrared light,which could kill tumor cells. Conclusion PLPN could emit red fluorescence,and could target-accumulated in tumor site and conduct the in vivo image,which has significant photothermai conversion properties in vivo and in vitro, and can effectively inhibit the tumor growth in vivo.

关 键 词：聚乙烯醇 脱镁叶绿酸 纳米粒子 光热转换 卵巢肿瘤 

分 类 号：R943[医药卫生—药剂学]