石墨烯-壳聚糖-纳米金修饰的免疫传感器应用于1-芘丁酸的检测研究  

Study on Graphene-Chitosan-Nano-Au Modified Amperometric Immunosensor for the Determination of 1-Pyrenebutyric Acid

作　　者：许贺[1] 许双姐[1] 柳建设[1] 金利通 

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]华东师范大学化学与分子工程学院,上海200062

出　　处：《分析科学学报》2017年第6期757-762,共6页Journal of Analytical Science

基　　金：中央高校科研业务费专项基金(No.17D111310);国家重点研发计划(No.2016YFC0400501)

摘　　要：本文采用石墨烯(GS)-壳聚糖(CS)-纳米金(Nano-Au)复合材料修饰玻碳电极,构建性能良好的生物识别界面,制备一种无标记的电流型免疫传感器,并应用于1-芘丁酸(PBA)的高灵敏检测。研究结果表明,GS、CS和Nano-Au的协同作用,极大地提高了anti-PAHs抗体在电极表面的覆盖量,从而提高了免疫传感器的灵敏度和检测性能。采用示差脉冲伏安法(DPV)对PBA进行检测,PBA在0.001~10ng/mL和10~200ng/mL浓度范围内与峰电流值呈良好的线性关系,检出限为0.001ng/mL。该传感器应用于实际水样中痕量PBA的检测,加标回收率为90%~105%。A simple, highly sensitive, and label-free electrochemical amperometrie immunosensor wasdeveloped based on graphene （GS）, chitosan （CS） and Nano-Au composites modified glassy carbonelectrode for the determination of 1-pyrenebutyric acid （PBA）. The morphology and electrochemicalproperties of the immunosensors were characterized by transmission electron microscopy and cyclicvoltammetry in detail. The study displayed that the amount of immobilization antibody-PAHs wasremarkably increased due to the synergistic effect of GS, CS and Nano-Au, resulting in that thesensitivity and detective performance of the proposed immunosensors were significantly enhanced. Theamperometric response was proportional to the PBA in the range from 0. 001 to 10 and from 10 to 200ng/mL with a detection limit of 0. 001 ng/mL. The proposed immunosensor was applied to detect tracePBA in real water samples,and the recoveries for spiking experiments were 90%-105% with satisfactoryresults.

关 键 词：石墨烯 电流型免疫传感器 多环芳烃 电化学检测 脉冲伏安法 

分 类 号：O657.1[理学—分析化学]