两种DNA分子同时检测的逻辑门及半加器的构建  

Construction of Logic Gates and Half Adder for Simultaneous Detection of Two DNA Molecules

作　　者：晋晓勇[1] 张笛[1] 蒙丽君 许凯歌 雷杰 彭娟[1] 

机构地区：[1]宁夏大学化学化工学院,省部共建煤炭高效利用与绿色化工国家重点实验室,宁夏银川750021

出　　处：《分析科学学报》2017年第6期763-768,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21365016,21765017);西部一流大学建设重大创新项目(No.2KZD2017003)

摘　　要：本研究利用滴涂法和电沉积法构建了氧化石墨烯/金纳米粒子复合膜修饰电极(GCE/GO/AuNPs),基于目标DNA和探针结合前后探针在电极表面构型的变化导致电化学信号变化,实现对大肠杆菌(E.coli)DNA和沙门氏菌(Sal)DNA的智能识别。以目标物为输入信号,分别以Σ|ΔI|和|ΔIMB/ΔIFc|为输出,构建了"AND"型和"XOR"型DNA分子逻辑门,提出了一种新型的可用于逻辑运算的半加器模型。采用方波伏安法(SWV)检测两种标记探针电流变化值Σ|ΔI|,其与E.coli DNA和Sal DNA浓度的对数值在1.0×10^(-13)~1.0×10^(-8) mol·L^(-1)范围内呈现良好的线性关系,检出限(S/N=3)分别为3.2×10^(-14) mol·L^(-1)和1.7×10^(-14) mol·L^(-1)。In this work, the composite film modified glassy carbon electrode （GO/AuNPs/GCE） wasprepared by drop coating and electrodeposition method. E. coli DNA and Sal DNA can be detectedsimultaneously and intelligently due to the current change when target oligonucleotides reacted withprobes. The ＂AND＂ and ＂XOR＂ DNA molecular logic gates were constructed with the target DNAs asthe inputs and Y lAII and IAIMB/AIFcl as different outputs. Correspondingly, a new half-addercalculation model for logic operation was proposed. The current sum [ All of two probes detected bySWV was linear to the logarithm value of concentraion for E. coli DNA and Sal DNA in the range of 1.0X10 la-1.0X10-8 mol. L-1,and the detection limit was 3.2X 10-14 mol. L-1 and 1.7X 10-14mol. L-1（S/N = 3 ）, respectively.

关 键 词：DNA分子逻辑门 电化学检测 半加器 生物传感器 

分 类 号：O657.1[理学—分析化学]