Eu^(3+)荧光探针构建及对牛奶中盐酸四环素残留的测定  

Determination of Tetracycline Hydrochloride in Milk Based on Eu^(3+) Fluorescent Probe

作　　者：高文慧 王兆群[1] 张勇[1] 泮俊青 尹洪宗[1] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《分析科学学报》2017年第6期822-826,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.31171953)

摘　　要：本文构建了Eu^(3+)-苯甲酸(BA)-邻菲啰啉(Phen)三元配合物体系荧光探针,建立了一种检测牛奶中盐酸四环素残留的新方法。对三元配合物体系进行优化,确定Eu^(3+)、BA、Phen的最佳浓度配比为1∶1.5∶2,最佳检测时间为20min。该方法检测盐酸四环素的线性范围为1.0×10^(-6)~5.0×10^(-5) mol/L,检测限为1.8×10^(-7) mol/L。利用该荧光探针对处理后的牛奶进行加标回收实验,其回收率范围为99.5%~108%,平均回收率为103.8%。实验证明该方法科学可行,对盐酸四环素具有较高的选择性。A novel method for the determination of tetracycline hydrochloride in the milk by using Eua＋-phenanthroline（Phen） as fluorescent probe was developed. Through optimizing the probe, the bestconcentration ratio of Eua＋ , BA, Phen was 1;1.5;2, the best detection time was 20 min. The fluorescenceintensity showed good linear relationship with tetracycline hydrochloride concentration ranged from 1.0X 10 6 to 5.0 X 10.2 mol/L with a detection limit of 1.80 X 10 7 mol/L. The Eu3＋-BA-Phen fluorescentprobe was utilized to detect tetracycline hydrochloride in the treated milk samples with the averagerecovery of 103.8 %. The results demonstrated that the method was feasible and had high selectivity totetracycline hydrochloride.

关 键 词：Eu3+ 荧光探针 盐酸四环素 苯甲酸 

分 类 号：O657.39[理学—分析化学]