核桃花粉萌发适宜培养基的筛选  

Screening of Optimum Medium for Pollen Germination of Walnut

作　　者：赵爽[1] 苏彦苹 李寒[1,3] 张雪梅 范舒畅[1] 齐国辉 

机构地区：[1]河北农业大学林学院,河北保定071000 [2]廊坊师范学院生命科学学院,河北廊坊065000 [3]河北省核桃工程技术研究中心,河北临城054300

出　　处：《北方园艺》2017年第23期42-47,共6页Northern Horticulture

基　　金："十二五"国家科技支撑计划资助项目(2013BAD14B03);河北省科技支撑计划资助项目(16236810D)

摘　　要：以早实核桃品种"绿岭"和"绿波"花粉为试材,采用离体培养方法,研究了培养基中不同比例蔗糖、硼酸、氯化钙对花粉萌发的影响,以明确核桃花粉萌发的适宜条件,为核桃花粉萌发找到适宜的培养基提供参考依据。结果表明:"绿岭"和"绿波"核桃花粉在蔗糖浓度为160g·L^(-1)时的发芽率最高,分别为48.09%和57.23%;"绿岭"和"绿波"花粉萌发最适宜的硼酸浓度分别为0.10、0.07g·L^(-1);2个品种的花粉萌发最适宜的氯化钙浓度为0.20g·L^(-1)。适宜"绿岭"核桃花粉萌发的最佳培养基为蔗糖160g·L^(-1)、硼酸0.10g·L^(-1)、氯化钙0.20g·L^(-1),在此培养基下"绿岭"核桃花粉萌发率达到61.06%;适宜"绿波"核桃花粉萌发的最佳培养基为蔗糖160g·L^(-1)、硼酸0.07g·L^(-1)、氯化钙0.20g·L^(-1),在此培养基下"绿波"核桃花粉萌发率为72.71%。Pollens of precocious walnut varieties ＇Lyuling＇ and ＇Lyubo＇ were used by culture method in vitro to study the effects of different proportion of sucrose, in order to determine the appropriate condition for walnut pollen germination and find out optimum medium, boric acid, calcium chloride on pollen germination. The results showed that the pollen germination rates of ＇ Lyuling＇ and ＇ Lyubo~ on the medium with sucrose concentration of 160 g ~ L-1 were the highest, which were 57.23~./00 and 48. 09 ~//00, respectively. The optimum boric acid concentrations for pollen germination of ＇Lyuling＇ and ＇Lyubo~ were 0.10 g ·L^-1 and 0.07 g·L^-1 ;the optimum calcium chloride concentration for pollen germination of the two varieties was 0. 20 g · L-1;the optimum medium for ＇Lyuling＇ walnut pollen germination was as follows,160 g · L^-1 sucrose,0.10 g·L^-1 boric acid,0.20 g · L^-1 calcium chloride,and the pollen germination rate of ＇Lyuling＇ walnut on this medium reached 61.06% tlae opUmum medium for ＇Lyubo＇ walnut pollen germination was as follows, 160 g·L-1 sucrose,O. 07 g·L^-1 boric acid,O. 20 g·L^-1 calcium chloride,and the pollen germination rate of ＇Lyubo＇ walnut on this medium reached 72. 71%.

关 键 词：核桃 花粉 培养基 

分 类 号：S664.103.6[农业科学—果树学]