COS-g-PEI修饰纳米银粒子的制备及其抗菌性  

Synthesis and Antimicrobial Property of COS-g-PEI Functionalized Silver Nanoparticles

作　　者：徐国园 孙熠昂 宗传晖 李爱香[1] 吕滋建[1] 

机构地区：[1]山东理工大学材料科学与工程学院,山东淄博255049

出　　处：《精细化工》2017年第12期1340-1344,共5页Fine Chemicals

基　　金：国家自然科学基金项目(51303096)~~

摘　　要：首先以离子液体1-丁基-3-甲基咪唑氯盐([BMIM]Cl)为反应介质和催化剂,羰基二咪唑为偶联剂,均相合成了壳寡糖接枝聚乙烯亚胺共聚物(COS-g-PEI);其次,以COS-g-PEI为稳定剂,硝酸银为前驱体,硼氢化钠为还原剂,采用原位还原法制备了COS-g-PEI修饰纳米银复合粒子(Ag NPs@-COS-g-PEI)。采用IR、~1HNMR对聚合物结构进行表征,结果证实成功合成了COS-g-PEI接枝共聚物;UV-Vis、TEM结果表明,成功制备了稳定的Ag NPs@-COS-g-PEI复合粒子。通过滤纸片法和牛津杯法测试了COS-g-PEI及Ag NPs@-COS-g-PEI对金黄色葡萄球菌和大肠杆菌的抗菌性能,Ag NPs@-COS-g-PEI的抑菌圈直径均比COS-g-PEI大,结果表明,Ag NPs与COS-gPEI有机结合大大提高了抗菌性能。A chitosan oligosaccharide（ COS）-graft-polyethylene imine copolymer（ COS-g-PEI） was first synthesized by using ionic liquid 1-butyl-3-methyl imidazolium chloride（ [BMIM]Cl） as a solvent and catalyst,and 1,1-carbonyldiimidazole as a coupling reagent. Then,COS-g-PEI functionalized silver nanoparticles（ Ag NPs@-COS-g-PEI） were prepared by in situ reduction of silver nitrate with COS-gPEI as a stable agent,and sodium borohydride as a reducing agent. The results of IR and ~1HNMR spectrometry showed that COS-g-PEI was prepared successfully. And the results of UV-Vis and TEM showed that the stable Ag NPs @-COS-g-PEI composite nanoparticles were obtained. Finally,the antimicrobial properties of COS-g-PEI and Ag NPs @-COS-g-PEI against staphylococcus aureus and escherichia coli were evaluated by Kirby-Baner test and oxford cup method. The results revealed that Ag NPs @-COS-g-PEI showed longer diameters of inhibiting zone against staphylococcus aureus and escherichia coli than COS-g-PEI,which indicated that the combination of COS and Ag NPs could greatly improve the antibacterial ability of the nanoparticles.

关 键 词：壳寡糖 聚乙烯亚胺 接枝共聚物 纳米银粒子 抗菌性能 功能材料 

分 类 号：TB383[一般工业技术—材料科学与工程]