重庆地区汉族人群KIR基因多态性研究  

The KIR gene diversity in Chongqing Han blood donors

作　　者：黄霞[1] 王珏[2] 欧国进[2] 王芳[1] 李小红[1] 廖红梅[1] 张涛[1] 毛伟[1] 

机构地区：[1]重庆市血液中心,重庆400015 [2]中国医学科学院输血研究所

出　　处：《中国输血杂志》2017年第10期1092-1095,共4页Chinese Journal of Blood Transfusion

基　　金：重庆市科委自然基金资助项目(CSTC;2010BB5339)

摘　　要：目的研究重庆地区汉族人群KIR基因及基因型多态性特点。方法采集316名重庆地区汉族无偿献血者的外周血样,应用多重PCR-SSP方法作KIR基因分型;根据等位基因频率网络数据库(AFND)中的ID号命名检测到的基因型及单体型组成,以Spss17.0软件对人群间KIR基因频率作卡方检验。结果 316名重庆地区汉族献血者个体均含有KIR基因3DL2、3DL3;2DL4、2DL1、3DP1、2DP1、2DL3、3DL1较为常见,频率分别为94.1%、92.0%、92.0%、90.3%、87.4%、76.8%;频率较低(<30%)的基因有2DL5、2DS1、3DS1、2DS5、2DL2、2DS2和2DS3。共发现24种KIR基因型,其中以ID号为1、2和8的基因型最为常见,频率分别为54.4%(172/316)、15.2%(48/316)和6.3%(20/316),还有1种基因型无法在AFND中查出其基因型。重庆地区汉族献血者携带的KIR各基因中分别有8个和7个基因的频率与南非圣族人和高加索人明显不同(P<0.05),有5个与乌市维族人明显不同(P<0.05),仅有1个与拉萨藏族有明显不同(P<0.05),而与四川汉族、云南汉族、浙江汉族、江苏汉族和韩国人均无明显差异(P>0.05)。结论重庆地区汉族人群的KIR基因分析既含丰富的多态性,又具有自身的特点,与四川、云南等中国南方汉族人群一致。Objective To analyze the diversity of killer cell immunoglobulin-like receptor（ KIR） genes and genotypes in Chongqing Han blood donors. Methods A total of 316 Chongqing Han volunteer blood donors were selected and genotyped by Multiplex PCR-SSP. The haplotypes and genotypes were designated according the Allele Frequency Net Database（ AFND）. χ2 tests were performed by KFsoftware 1. 61. Results Among Chongqing Han blood donors,KIR 3 DL2、3 DL3 were found in all samples,genes 3 DL2、3 DL3; 2 DL4、2 DL1、3 DP1、2 DP1、2 DL3、3 DL1 present high frequencies at 94. 1%、92. 0%、92. 0%、90. 3%、87. 4% and 76. 8% respectively,gene frequencies of 2 DL5、2 DS1、3 DS1、2 DS5、2 DL2、2 DS2 and 2 DS3 were less than 30. 0%. A total of 24 KIR genotypes were identified,in which the genotypes named ID1（ 54. 4%）,ID2（ 15. 2%）and ID8（ 6. 3%） were predominant,one genotype couldn＇ t assign ID according AFND. The carrier frequencies of 8 KIR genes in Chongqing Han blood donors were different from that of South African San and Caucasian,5 were different from Urumqi uygur,only 1 was different from Lasa Tibetan,no difference was observed between China South Han and South Korea populations in all 17 KIR genes. Conclusion Chongqing Han blood donors show close characteristics with China South Han populations in KIR genes diversity.

关 键 词：重庆汉族 杀伤细胞免疫球蛋白样受体 多态性 基因型 献血者 

分 类 号：R457.1[医药卫生—治疗学]