云南楚雄地区彝族献血人群KIR基因多态性研究  

KIR genes diversity in Yi blood donors of Chuxiong

作　　者：王珏[1] 杨通汉[2] 蒋黎 车忠民[2] 纪欣[1] 罗臻[2] 欧国进[1] 苏品璨[2] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]云南昆明血液中心

出　　处：《中国输血杂志》2017年第10期1099-1102,共4页Chinese Journal of Blood Transfusion

基　　金：昆明市科技计划重点项目(昆科计字2013-04-02-AS-01-2141号);中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-024)

摘　　要：目的研究云南楚雄地区彝族献血人群KIR基因及基因型多态性特点。方法从云南地区无偿献血者中选取157名楚雄彝族健康自愿者,采用多重PCR-SSP方法进行KIR基因检测。根据http://www.allelefrequencies.net/中的ID号命名基因型和单体型组成。结果在云南地区楚雄彝族人群中,框架基因KIR3DL2、3DL3、2DL4和3DP1存在于所有个体;较为常见KIR基因有2DP1、2DL1、2DL3、3DL1和2DS4,其基因频率分别为0.920 2、0.920 2、0.861 8、0.774 3和0.760 6;频率较低的基因有KIR 2DS3、2DS2、2DL2、2DS5、3DS1、2DS1和2DL5,其基因频率分别为0.097 1、0.140 4、0.144 1、0.182 2、0.226 2、0.226 2和0.277 3;共发现19种KIR基因型,其中以ID号为1和2的基因型最为常见,频率为47.13%和16.56%。结论云南地区楚雄彝族人群KIR基因分布与中国南方汉族人群之间较一致,部分基因位点与韩国人、南非人,乌鲁木齐地区维吾尔族人和高加索地区人差异显著。Objective To analyze the diversity of killer cell immunoglobulin-like receptor genes and genotypes in Yunnan chuxiong Yi blood donors. Methods A total of 157 individuals were selected from Yi volunteer blood donors in Yunnan chuxiong,and genotyped by Multiplex PCR-SSP. The genotypes and haplotypes were designated according http：//www. allelefrequencies. net/. Results Among the Yi population,framework genes 3 DL2,3 DL3,2 DL4 and 3 DP1 were found in all samples,genes 2 DP1,2 DL1,2 DL3,3 DL1 and 2 DS4 were with high frequencies（ 0. 920 2,0. 920 2,0. 861 8,0. 774 3 and 0. 760 6,respectively）,genes KIR 2 DS3,2 DS2,2 DL2,2 DS5,3 DS1,2 DS1 and 2 DL5 were with low frequencies（ 0. 0971,0. 140 4,0. 144 1,0. 182 2,0. 226 2,0. 226 2 and 0. 277 3,respectively）. A total of 19 KIR genotypes were identified,in which the genotypes named ID1（ 47. 13%） and ID2（ 16. 56%）are predominant. Conclusion Yunnan chuxiong Yi population have the same feature as southern Han populations on KIR gene diversity,part of the genes frequencies significantly different from South Korea、South Africa,Urumqi uygur and Caucasian population.

关 键 词：杀伤细胞免疫球蛋白样受体 云南彝族 基因频率 基因型 

分 类 号：R457.11[医药卫生—治疗学]