美洲茶藨子查耳酮合成酶基因电子克隆和生物信息学分析  

In Silico Cloning and Bioinformatics Analysis of Chalcone Synthase Gene from Ribes Americanum

作　　者：任伟超 温东[2] 赵锐[2] 马伟[2] 

机构地区：[1]伊春林业科学院,黑龙江伊春153000 [2]黑龙江中医药大学药学院

出　　处：《中国药师》2017年第12期2106-2109,共4页China Pharmacist

基　　金：国家公益性行业专项寒温带道地药材仿生态培育技术研究与示范项目(编号:201404718)

摘　　要：目的:对美洲茶藨子查耳酮合成酶基因进行电子克隆及生物信息学分析。方法:以黑茶藨子查耳酮合成酶基因序列和美洲茶藨子表达序列标签为基本材料(探针),运用CAP3在线软件进行序列拼接,并对其进行了生物信息学分析。结果:美洲茶藨子查耳酮合成酶基因全长1 423 bp,包含1 173 bp的开放阅读框,编码390个氨基酸,该蛋白是定位于细胞质的亲水性蛋白,存在三处跨膜区,二级结构主要由α-螺旋构成。结论:研究结果有利于美洲茶藨子查耳酮合成酶基因的分子作用机制等方面的研究。Objective： To study the in silico cloning and bioinformatics of chalcone synthase gene from Ribes americanum. Meth- ods： Ribes nigrum chalcone synthase sequence and Ribes americanum were cloned by retrieving the EST database as the querying probe, which were assembled by CAP3 sequence assembly program, and the bioinformatics database and related software were used to predict the structure and perform the function analysis. Results： Bioinformatical analysis showed chalcone synthase gene encoded 1 423 bp and contained a 1173bp ORF, the protein was with 390 amino acid, which was a hydrophilic protein located in cytoplasm including three transmembrane regions with the secondary structure composed of alpha helix. Conclusion： The study is helpful to the further explanation for the molecular function mechanism of chalcone synthase gene from Ribes americanum.

关 键 词：美洲茶藨子 查耳酮合成酶 电子克隆 生物信息学 

分 类 号：Q949.751.2[生物学—植物学]