正交试验法优化五味子总木脂素的提取工艺  

Optimization of Extraction Process for Total Lignans from Fructus Chinensis by Orthogonal Test

作　　者：李莹丽[1] 朱志军[1] 张楠楠[1] 邓浩[1] 高松[1] 李颖 

机构地区：[1]河南中医药大学药学院,郑州450008

出　　处：《中国药师》2017年第12期2139-2142,共4页China Pharmacist

基　　金：河南省科技攻关计划(编号:162102310450)

摘　　要：目的:优化五味子总木脂素提取工艺。方法:采用正交试验法,以五味子醇甲、甲素及乙素的含量为五味子总木脂素的含量,与浸膏含量综合加权评分,考察乙醇浓度、提取时间、乙醇倍数、提取次数对五味子总木脂素的影响;采用HPLC法:华普Unitary C18柱(250 mm×4.6 mm,5μm),流动相为甲醇(A)-水(B),梯度洗脱(0~10 min,A为70%;10~13 min,A为70%~85%;13~30 min,A为85%);检测波长:217 nm;柱温:30℃;流速:1.0 ml·min^(-1),进样量:10μl。结果:五味子总木脂素提取的最佳工艺条件为:14倍量的85%乙醇提取3次,每次2.5 h,五味子总木脂素提取率为1.10%,浸膏量为22.99%。结论:五味子总木脂素提取工艺稳定可靠,为生脉分散片新剂型改革提供了科学基础。Objective： To optimize the extraction process of the total lignans from Fructus chinensis. Methods： The comprehensive weighted score method for the extraction rate and the extraction content was regarded as the index by orthogonal experimental method, the effects of ethanol concentration, extraction duration, amount of ethanol and extraction times on the extraction of the total lignans from Fructus chinensis were studied. The chromatographic separation was performed on a Unitary Cis column （250 mm×4.6 mm,5μm）. Methanol（ A）-water（ B） was used as the mobile phase （0-10 min：70% A; 10-13 min：70% A-85% A; 13-30 min：85% A）. The detection wavelength was set at 217 nm, the column temperature was maintained at 30℃ ,the flow rate was 1.0 ml·min^-1 and the sample size was 10 μl. Results： The extraction conditions of the total lignans were as follows： 14-fold amount of 85% ethanol was used for 3 times extraction with 2.5 h for each time. Under the above conditions, the extraction rate of Schisandra lignans was 1.10%, and the extraction content was 22.99%. Conclusion： The extraction and purification technology for Schisandra lignans and the total poly- saecharides is stable and reliable, which provides scientific basis for the reform of Shengmai new dosage forms.

关 键 词：五味子木脂素 正交试验法 醇提 

分 类 号：R284.2[医药卫生—中药学]