脊髓压迫性损伤大鼠海马和脊髓内BDNF的表达变化  

Changes of BDNF expression in hippocampus and spinal cord of rats after compression-induced spinal cord injury

作　　者：黄思琴[1,2] 张安宁 孙善全[2] 王娟 

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]重庆医科大学神经科学平台,重庆400016 [3]重庆医科大学附属永川医院神经内科,重庆402160

出　　处：《基础医学与临床》2017年第12期1681-1685,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81403466);重庆市体育局科研项目(B201408);重庆市渝中区科技计划项目(20140117);重庆市卫计委项目(ZY20150250;2015MSXM059);永川区科委项目(2015nc5007)

摘　　要：目的探讨脑源性神经营养因子(BDNF)在脊髓压迫性损伤后的表达变化及作用。方法用自行设计的方法制作脊髓压迫模型,免疫荧光检测BDNF在星形胶质细胞、神经元和上下行轴突的表达;WB检测BDNF在大鼠海马及脊髓的表达。结果随着时间延长,受损部位的BDNF+-GFAP荧光强度逐渐增强;BDNF+-Tuj1荧光强度逐渐减弱;受损部位相邻上下段的BDNF+-NF200比受损部位的明显增强。海马和脊髓受损中心相邻的上、下段的BDNF蛋白表达在损伤后1d达到峰值,然后逐渐下降(P<0.05);而脊髓受损中心的BDNF蛋白表达逐渐下降(P<0.05)。结论脊髓损伤后,BDNF的表达下调,随伤后时间呈一定规律变化,是引起受损部位神经元表达下降的原因之一。Objective To observe changes of BDNF expression in hippocampus and spinal cord of rats after compression-induced spinal cord injury. Methods The CSCI model was established with a institution-made device.BDNF in astrocyte,neuron,antegrade and retrograde axons were detected by immunofluorescence. The expression changes of BDNF in hippocampus,lesion centre,the superior segment and the inferior segment which were adjacent to the lesion centre were also determined by Western blot methods. Results The fluorescence intensity of BDNF＋-GFAP in lesion centre was increased,while BDNF＋-Tuj1 was decreased with time after CSCI. BDNF＋-NF200 in superior and inferior segment of lesion was increased. The expression of BDNF in hippocampus,the superior segment and the inferior segment close to the lesion centre reached its peak on the 1 st day and then decreased after CSCI（ P〈0. 05）. The expression of BDNF in lesion centre decreased gradually（ P〈0. 05）. Conclusions Decreasing level of BDNF is regular and may lead to neuronal reduction with compressing time going.

关 键 词：脊髓压迫性损伤 脑源性神经营养因子 神经元 星形胶质细胞 

分 类 号：R651.2[医药卫生—外科学]