驯化后的聚糖菌对NO_2^--N和NO_3^--N内源反硝化速率的影响  

Effect of endogenous denitrification rate of domesticated GAOs on NO_2^--N and NO_3^--N

作　　者：贾淑媛[1] 王淑莹[1] 赵骥[1] 李夕耀[1] 张琼[1] 彭永臻[1] 

机构地区：[1]北京工业大学国家工程实验室北京市水质科学与水环境恢复工程重点实验室,北京100124

出　　处：《化工学报》2017年第12期4731-4738,共8页CIESC Journal

基　　金：国家自然科学基金项目(51578014);北京市教委资助项目~~

摘　　要：在序批式(sequencing batch reactor,SBR)反应器中,通过分段厌氧-好氧(厌氧后排水)运行方式,在以葡萄糖为碳源、P/C比小于2/100的条件下,成功实现了聚糖菌(glycogen accumulating organisms,GAOs)的驯化富集,厌氧段磷酸盐的释放量(phosphorus release amounts,PRA)稳定在1.0 mg·L-1以内,胞内糖原(glycogen,gly)含量是初始阶段的1.2倍。驯化后的GAOs分别以NO_2^--N、NO_3^--N为电子受体经厌氧-缺氧运行方式,可进行内源反硝化反应过程。GAOs在内源反硝化过程中依次利用胞内的聚β-羟基戊酸酯(poly-β-hydroxyvalerate,PHV)、聚β-羟基丁酸酯(poly-β-hydroxyvalerate,PHB)和gly作为内碳源。在22℃时,反硝化聚糖菌(denitrifying glycogen accumulating organisms,DGAOs)以NO_2^--N、NO_3^--N为电子受体平均比内源反硝化速率分别为0.067 g N·(g VSS)-1·d-1、0.023 g N·(g VSS)-1·d-1,常温短程内源反硝化速率约是全程内源反硝化速率的3倍。Glycogen accumulating organisms（GAOs） has been enriched in the anaerobic-aerobic sequencing batch reactor（AO-SBR）（drained after anaerobic） with glucose as the carbon source and the P/C ratio less than 2/100. PRA was less than 1.0 mg·L-1. The content of gly was 1.2 times the initial stage of GAOs enrichment. The domesticated GAOs cultured in anaerobic-anoxic operation could proceed endogenous denitrification reaction with NO2--N and NO3--N as endogenous carbon source. GAOs used intracellular poly-β-hydroxyvalerate（PHV）, poly-β-hydroxyvalerate（PHB） and glycogen（gly） in turns as carbon source in endogenous denitrification process. The average endogenous denitrification rate of DGAOs using NO2--N and NO3--N as electron acceptors were 0.067 g N·（g VSS）-1·d-1 and 0.023 g N·（g VSS）-1·d-1 at 22℃, respectively. Short-range endogenous denitrification rate was about three times as much as endogenous denitrification rate at normal temperature.

关 键 词：聚糖菌 厌氧 需氧 曝气 富集 内源反硝化 

分 类 号：X703.1[环境科学与工程—环境工程]