清热解毒化浊片对肠源性内毒素血症肝损伤大鼠肝组织TNF-α及小肠ZO-1蛋白的影响  

Effects of Qingre Jiedu Huazhuo Tablet(清热解毒化浊片) on Hepatic Tissue TNF-α and Intestinal ZO-1 Protein Expressions of Liver Injury Rats with Intestinal Endotoxemia

作　　者：赵国荣[1] 戴玉微 陈研焰 崔玉晖 毛娅男[1] 唐菲[1] 黎亚 

机构地区：[1]湖南中医药大学,湖南长沙410007 [2]湖南中医药高等专科学校,湖南株洲412012 [3]威海市中医院,山东威海264200

出　　处：《中国中医药科技》2017年第6期699-703,共5页Chinese Journal of Traditional Medical Science and Technology

基　　金：湖南省教育厅重点项目资助(12A101);湖南中医药大学中医药临床基础重点学科资助

摘　　要：目的:观察清热解毒化浊片对肠源性内毒素血症肝损伤大鼠炎性因子TNF-α及小肠紧密连接蛋白-1(zonula occluden-1,ZO-1)(中文名称)的影响。方法:以高脂饲料和52%Vol.白酒喂养建立肠源性内毒素血症肝损伤模型,造模成功后,随机分为治疗1组、治疗2组和模型组,模型组用白酒及蒸馏水灌胃、治疗1组用白酒及清热解毒化浊片悬浊液灌胃、治疗2组用蒸馏水及清热解毒化浊片悬浊液灌胃。经治疗1个月后检测肝功能(ALT、AST、ALP、GGT)及血浆内毒素水平,观察肝组织及小肠组织病理学改变,免疫组化法检测肝组织TNF-α及小肠组织ZO-1蛋白的表达。结果:与正常组比较,模型组血浆内毒素水平明显升高(P<0.01),ALT、AST、GGT水平均明显升高(P<0.01),ALP水平升高(P<0.05),肝组织TNF-α明显升高(P<0.01),小肠组织ZO-1表达降低(P<0.05);与模型组比较,治疗2组内毒素水平明显降低(P<0.01),治疗1组及2组ALT、AST、GGT水平均明显降低(P<0.01),ALP水平降低(P<0.05),肝组织TNF-α表达降低(P<0.05),小肠组织ZO-1表达升高(P<0.05)。光镜下观察,治疗1组及2组大鼠肝组织及小肠组织炎性浸润情况明显改善。结论:清热解毒化浊片可以通过上调肠组织ZO-1蛋白的表达,维持肠黏膜屏障功能,减少内毒素吸收,降低促炎因子TNF-α的释放,从而在一定程度上终止内毒素血症与肝损伤的恶性循环。Objective：To observe the effect of Qingre Jiedu Huazhuo Tablet on hepatic tissue TNF-α and intestinal protein ZO-1 of liver injury rats with intestinal endotoxemia（IETM）.Methods：Liver injury rat models with IETM were established by feeding with high-fat diet and 52% Vol.liquor.The successful rat models were randomly divided into treatment group 1,treatment group 2 and model group.Liquor and distilled water were administered by gavage in model group,liquor and Qingre Jiedu Huazhuo Tablet suspension liquid were administered by gavage in treatment group 1,distilled water and Qingre Jiedu Huazhuo Tablet suspension liquid were administered by gavage in treatment group 2.After 1-month treatment,liver functions（ALT,AST,ALP,GGT） and plasma endotoxin level was tested through blood sampling,liver and intestine histopathological changes were observed,and immunohistochemistry was used to test the expression of TNF-α in liver tissues and protein ZO-1 in intestinal tissues.Results：Compared with normal group,in the model group,endotoxin level increased significantly（P〈0.01）,ALT,AST,GGT levels increased significantly（P〈0.0）,ALP level increased（P〈0.05）,TNF-α expression in hepatic tissue increased significantly（P〈0.01） and intestinal ZO-1 protein expression decreased（P〈0.05）.Compared with the model group,endotoxin level decreased significantly（P〈0.01） in treatment group 2;ALT,AST,GGT levels all decreased significantly（P〈0.01）,liver TNF-α expression decreased（P〈0.05）,intestine ZO-1 expression increased（P〈0.05）,in treatment group 1 and 2.Observed under light microscope,inflammatory invasions in liver and intestinal tissues of rats in treatment group 1 and 2 improved markedly.Conclusion：Qingre Jiedu Huazhuo Tablet can maintain the barrier function of intestinal mucosal,reduce endotoxin absorption and the release of pro-inflammatory cytokine TNF-α by increasing protein ZO-1 expression in intestinal tissues,terminating the vicious cycle of endotoxemia and liver

关 键 词：清热解毒化浊片 肠源性内毒素血症 TNF-Α ZO-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]