一测多评法测定毛郁金中5种成分  

Quantitative analysis on content of different components in Curcumae Aromaticae Radix by QAMS

作　　者：郭青玲 周福军[2] 单淇[2] 黄景华 王习著 华洁[2] 王淼[2] 侯文彬[1] 

机构地区：[1]天津中医药大学,天津300070 [2]天津药物研究院,天津300193 [3]桂林八加一药业股份有限公司,广西桂林541000

出　　处：《药物评价研究》2017年第9期1274-1278,共5页Drug Evaluation Research

基　　金：国家新药创制项目(2014ZX09101-021-001)

摘　　要：目的建立毛郁金药材中不同成分同时测定的一测多评法。方法以毛郁金中莪术二酮为指标,建立莪术二醇、ocathydro-1,4-dihydroxy-1,4-dimethyl-7-(propan-2-ylidene)azulen-5(1H)-one、原莪术醇和姜黄素的相对校正因子,在考察相对校正因子的重现性后,进一步采用外标法对一测多评法所计算出的成分含量进行验证。结果 10批毛郁金药材中,各成分采用相对因子计算的含量与采用外标法计算的结果无显著差异。结论以莪术二酮为参照物,采用一测多评测定和计算其他4种成分含量的方法是可行的,结果较为准确。Objective To develop a method of quantitative analysis of multi-components by single marker （QAMS） forsimultaneously determining five compounds in Curcumae Aromaticae Radix. Methods An HPLC method was developed asQAMS to determine curcuma diol, ocathydro-l,4-dihydroxy-l,4-dimethyl-7-（propan-2-ylidene）azulen-5（1H）-one, original curcumoland curcumin in Curcumae Aromaticae Radix, using curdione as intermal reference substance, and the relative correction factor（RCF） of the four components was determined by HPLC with good reproducibility. Their contents in 10 batches of samples, collectedfrom different areas, were determined by both external standard method and QAMS. Result No significant differences were foundin the quantitative results of four compounds in l0 batches of Curcumae Aromaticae Radix determined by external standard methodand QAMS. Conclusion It is feasible and suitable to evaluate the quality of Curcumae Aromaticae Radix by QAMS.

关 键 词：一测多评 毛郁金 莪术二酮 莪术二醇 ocathydro- 1 4-dihydroxy- 1 4-dimethyl-7-(propan-2-ylidene)azulen-5 ( 1H)-one 原莪术醇 姜黄素 

分 类 号：R965.2[医药卫生—药理学]