氧化苦参碱对HepG-2与A549细胞增殖抑制与促凋亡活性比较研究  

Comparative study of proliferative inhibition and apoptosis promoting activity on HepG-2 and A549 by oxymatrine

作　　者：王淑静[1] 任爽[1] 张家宁[1] 孙微微[1] 袁天宇 马琳 

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《药物评价研究》2017年第11期1545-1549,共5页Drug Evaluation Research

基　　金：哈尔滨市科技创新人才研究专项基金资助(2016RQQXJ095);哈尔滨商业大学大学生创新创业训练计划国家级项目(201710240001)

摘　　要：目的研究氧化苦参碱在体外对人肺癌细胞(A549)、人肝癌细胞(HepG-2)增殖抑制与促凋亡活性,并进行比较。方法通过MTT比色法和生长曲线法比较氧化苦参碱对两种肿瘤细胞增殖活性的抑制作用,采用HE染色、Hoechst 33258荧光染色和透射电镜3种方法比较氧化苦参碱对两种肿瘤细胞形态的影响及促凋亡作用。结果 MTT结果显示,与对照组比较,氧化苦参碱200、400、800μg/m L浓度组A549、HepG-2细胞存活率均显著降低(P<0.05、0.01),半数抑制浓度(IC50)分别为1 055.45、774.11μg/m L;生长曲线结果显示,与对照组比较,氧化苦参碱组HepG-2细胞培养第4、5天及A549细胞第5天细胞数显著下降(P<0.05、0.01);在HE染色、Hoechst 33258荧光染色和透射电镜形态学观察实验中,氧化苦参碱对HepG-2细胞作用较明显,细胞数量明显减少,同时出现细胞绒毛减少、细胞核变小变圆、固缩等明显的细胞凋亡形态学特征,对A549细胞形态的影响较弱。结论氧化苦参碱具有抑制肿瘤细胞增殖和促进凋亡的作用,对HepG-2作用较强,对A549作用较弱,具有一定的特异性。Objective To study the proliferative inhibition and apoptosis promoting activity of oxymatrine on human lung cancer cells（A549） and human hepatocellular carcinoma cells（HepG-2） in vitro, and make a comparison. Methods Effect of oxymatrine on the proliferation activity of two kinds of tumor cells were compared by MTT method and growth curve method. HE staining, Hoechst 33258 fluorescent staining and transmission electron microscopy were used to compare morphological changes. Results MTT results showed that, compared with control group, the survival rate of oxymatrine 200, 400, and 800 μg/m L concentration group in A549 and HepG-2 cells decreased significantly（P〈0.05, 0.01）, half inhibitory concentration（IC50） were 1055.45 and 774.11 g/m L respectively; The growth curve showed that, compared with control group, the number of cells in oxymatrine group of HepG-2 cells cultured for fourth and fifth day and A549 cells cultured for fifth day decreased significantly（P〈0.05, 0.01）. Oxymatrine affected morphological changes of HepG-2 cells more significantly than A549 cells. The number of cells in the oxymatrine group was significantly reduced; And the cell villi was decreased, the nucleus was small and round, and the cell apoptosis morphological characteristics were obvious. Conclusion Oxymatrine can inhibit the proliferation cells and promote the apoptosis of tumor cells. It affected HepG-2 cells more obviously, which suggests that it may have different sensitivity to different tumor cells.

关 键 词：氧化苦参碱 抗肿瘤活性 人肝癌细胞(HepG2) 人肺癌细胞(A549) 增殖 凋亡 

分 类 号：R965.1[医药卫生—药理学]