益气活血通络方对糖尿病周围神经病变小鼠JNK信号通路蛋白及炎性因子的影响  

Influence of Yiqi Huoxue Tongluo Fang on JNK signal pathway and inflammatory factors in mice with diabetic peripheral neuropathy

作　　者：姚永传 申国明 江爱娟 王靓 叶树[1] 王柳[1] 

机构地区：[1]安徽中医药大学中西医结合临床学院,安徽230038 [2]安徽中医药大学研究生院

出　　处：《北京中医药大学学报》2017年第11期923-927,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81273646);安徽省自然科学基金项目(No.1608085MH232)~~

摘　　要：目的研究益气活血通络方对糖尿病周围神经病变模型小鼠(db/db小鼠)JNK信号通路蛋白及炎性因子的影响,探讨其对db/db小鼠周围神经的保护作用机制。方法 40只db/db小鼠随机分为5组:模型组、硫辛酸组和益气活血通络方高、中、低剂量(6.4、3.2、1.6 g/kg)组,另设8只db/m小鼠作为正常组。灌胃给药,1次/d,连续8周。末次给药后,测定小鼠足底热敏反应时间和运动神经传导速度(MNCV);酶联免疫吸附法(ELISA法)检测血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平;Western blot法检测背根神经节中c-jun氨基末端激酶(JNK)和磷酸化的c-jun氨基末端激酶(p-JNK)的蛋白表达。结果与正常组比较,模型组小鼠MNCV减慢,足底热敏反应时间延长,血清中IL-1β、TNF-α水平升高,背根神经节中JNK、p-JNK蛋白的表达增强,差异具有统计学意义(P<0.01或P<0.05)。与模型组比较,益气活血通络方能提高小鼠MNCV,缩短足底热敏反应时间,降低血清中IL-1β、TNF-α水平,一定程度下调背根神经节中JNK、p-JNK的蛋白表达(P<0.01或P<0.05)。结论益气活血通络方能抑制JNK信号转导通路的异常激活,减轻db/db小鼠炎症反应,从而保护周围神经的功能。ObjectiveTo study the influence of Yiqi Huoxue Tongluo Fang （Qi-Replenishing Blood- Activating Collateral-freeing Decoction ） on signal pathway of c-jun N-terminal kinase （JNK） and inflammatory factors in mice with diabetic peripheral neuropathy （db/db mice ）, and discuss its mechanism in protecting peripheral nerve. Methods The db/db mice （n = 40） were randomly divided into 5 groups： model group, lipoic acid group, high-dose Yiqi Huoxue Tongluo Fang group （6.4 g/kg, high-dose group）, mid-dose Yiqi Huoxue Tongluo Fang group （ 3.2 g/kg, mid-dose group） and low-dose Yiqi Huoxue Tongluo Fang group （ 1.6 g/kg, low-dose group）, and other db/m mice （ n = 8 ） were chosen into normal group. All groups were intragastrically given corresponding drugs once a day for 8 weeks. After the last administration of drugs, the plantar thermal response time and motor nerve conduction velocity （MNCV） were detected. The levels of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） were detected by using enzyme-linked immunosorbent assay （ELISA）. The protein expressions of JNK and phosphorylase JNK （p-JNK） in dorsal root ganglion （DRG） were detected by using Western blot method. Results Compared with normal group, MNCV was slower, plantar thermal response time was longer, levels of IL-1β and TNF-α increased, and protein expressions of JNK and p- JNK are stronger in model group （ P 〈 0.01 or P 〈 0.05 ）. Compared with model group, Yiqi Huoxue Tongluo Fang promoted MNCV, shortened plantar thermal response time, decreased levels of IL-1β and TNF-α and down-regulated protein expressions of JNK and p-JNK to some extent （ P 〈 0.01 or P 〈 0.05 ）. ConclusionYiqi Huoxue Tongluo Fang can inhibit the abnormal activation of JNK signal pathway and relieve inflammatory reaction to protect peripheral nerve in db/db mice.

关 键 词：益气活血通络方 DB/DB小鼠 糖尿病周围神经病变 JNK信号转导通路 炎性因子 

分 类 号：R285.5[医药卫生—中药学]