鹿茸多肽-PLGA复合膜提供周围神经再生微环境的实验研究  

Microenvironment of peripheral nerve regeneration supported by velvet antler polypeptides-PLGA compound membrane

作　　者：王克利 张静玲[2] 路来金[3] 

机构地区：[1]中国人民解放军火箭军总医院骨科,北京100088 [2]北京科技大学医院 [3]吉林大学第一临床学院

出　　处：《北京中医药大学学报》2017年第11期928-932,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30470833)~~

摘　　要：目的探讨鹿茸多肽(VAP)与聚乳酸(PLA)和聚羟基乙酸(PGA)共聚物(PLGA)形成的VAP-PLGA复合膜对大鼠坐骨神经再生的影响。方法健康雄性Wistar大鼠36只,随机分成4组,每组9只。将大鼠坐骨神经进行手术显露,假手术组神经游离后不进行处理;模型组神经切断后断端直接吻合;对照组神经切断吻合后包裹PLGA复合膜;治疗组神经切断吻合后包裹VAP-PLGA复合膜。术后第2、4、6周取材,分别行组织学、免疫组化观察和检测。结果在3个时相点,组织学:假手术组为正常神经轴突,治疗组有髓神经纤维数量明显多于模型组、对照组,轴突再生率和再生轴突成熟度明显高于模型组、对照组(P<0.05)。免疫组化染色:治疗组再生神经纤维轴突及髓鞘转化生长因子-β(TGF-β)、胰岛素样生长因子(IGF)抗原染色和抗原表达均较假手术组、模型组、对照组高(P<0.05)。结论 VAP-PLGA复合膜能够提供神经修复所需要的微环境和神经活性因子,从而促进周围神经再生,是理想的神经修复生物材料。Objective To investigate the influence of velvet antler polypeptides （VAP） -PLGA compound membrane formed from VAP and copolymer （PLGA） of polylactic acid （PLA） and polyglycolic acid （PGA） on regeneration of sciatic nerve in rats. Methods Healthy male Wistar rats （n = 36 ） were randomly divided into 4 groups （ each n = 9）. The sciatic nerve of rat was revealed by operation, and was not treated in sham-operation group after isolating the nerve and sewed up directly broken ends in model group after cutting the nerve. The sciatic nerve of rat was sewed up and wrapped up with PLGA compound membrane in control group and the nerve was sewed up and wrapped up with VAP-PLGA eompoundmembrane in treatment group. The samples were collected respectively on the 2nd , 4th and 6th weeks after the operation and given histological and immunohistochemical observation and detection. Results The histological observation showed that nervous axon was normal in sham-operation group at 3 time points. The quantity of medullated fibers was significantly more, and axon regeneration rate and maturity of regenerated axon were significantly higher in treatment group than those in model group and control group （P 〈 0.05 ） at 3 time points. The immunohistochemical detection showed that the antigen staining and antigen expressions of transforming growth factor β （TGF-β） and insulin-like growth factor （IGF） in regenerated nerve fiber axon and myelin sheath were significantly higher in treatment group than those in sham-operation group, model group and control group （ P 〈 0.05 ）. Conclusion VAP-PLGA compound membrane can provide a microenvironment and neuroactive factors required by neural restoration, and promote the regeneration of peripheral nerve, which is an ideal biological material for neural restoration.

关 键 词：鹿茸多肽 聚乳酸聚羟基乙酸共聚物 周围神经 神经再生 大鼠 

分 类 号：R285.5[医药卫生—中药学]