Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities  

作　　者：XIONG Jia GRACE Mary H ESPOSITO Debora KOMARNYTSKY Slavko WANG Fei LILA Mary Ann 

机构地区：[1]Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University [2]Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University,North Carolina Research Campus

出　　处：《Chinese Journal of Natural Medicines》2017年第11期816-824,共9页中国天然药物（英文版）

基　　金：supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD);State Forestry Administration of China(No.201104019)

摘　　要：The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract(B) and fractions(B1-B7) obtained by high-speed counter-current chromatography(HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes(α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7(total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g^(-1)), respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g^(-1)), respectively) showed significant activities against reactive oxygen species(ROS), nitric oxide(NO) production, and expression of pro-inflammatory genes interleukin-1β(IL-1β) and inducible nitric oxide synthase(iNOS) in a lipopolysaccharide(LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme(IC_(50) of 0.4-1.4 μg·mL^(-1)) than the pharmaceutical acarbose(IC_(50) 141.8 μg·mL^(-1))). B6 and B7(IC_(50) 17.6 and 11.7 μg·mL^(-1), respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose(IC_(50) 15.4 μg·mL^(-1))). Moreover, B extract, at 25 μg·mL^(-1), significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract（B） and fractions（B1-B7） obtained by high-speed counter-current chromatography（HSCCC） and evaluate their anti-inflammatory and carbolytic enzymes（α-glucosidase and α-amylase） inhibitory activities. Fractions B4, B5, B6, B7（total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g-1）, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g-1）, respectively） showed significant activities against reactive oxygen species（ROS）, nitric oxide（NO） production, and expression of pro-inflammatory genes interleukin-1β（IL-1β） and inducible nitric oxide synthase（iNOS） in a lipopolysaccharide（LPS）-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme（IC50 of 0.4-1.4 μg·mL-1） than the pharmaceutical acarbose（IC50 141.8 μg·mL-1））. B6 and B7（IC50 17.6 and 11.7 μg·mL-1, respectively） exhibited α-amylase inhibitory activity as efficacious as acarbose（IC50 15.4 μg·mL-1））. Moreover, B extract, at 25 μg·mL-1, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.

关 键 词：ACACIA mearnsii PROANTHOCYANIDINS ANTI-INFLAMMATORY Α-AMYLASE Α-GLUCOSIDASE 

分 类 号：R965[医药卫生—药理学]