利用SLAF-Seq-BSA定位矮秆波兰小麦矮化基因Rht-dp  

Mapping of Rht-dp Derived from Dwarf Polish Wheat(Triticum polonicum) via SLAF-Seq-BSA

作　　者：柴松岳 姚琴 程怡然 王超[1] 周永红[1] 王益[1] 

机构地区：[1]四川农业大学小麦研究所,成都611130

出　　处：《四川农业大学学报》2017年第4期459-464,475,共7页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金项目(31671688)

摘　　要：【目的】为了显著地缩小矮秆波兰小麦矮化基因Rht-dp的候选区域。【方法】借助SLAF-Seq-BSA(specific-locus amplified fragment sequencing and bulked segregant analysis)技术进行Rht-dp的关联分析。【结果】通过对亲本、极高和极矮池的SLAF-Seq分析,SLAF标签均匀分布在A和B基因组上,但仍有部分标签分布在D基因组或没有绑定在中国春基因组上。同时将Rht-dp定位在4B基因组上的154 766 751-343 858 300区域;结合前期SSR标记分析结果(99 826 939-250 584 662),最终将Rht-dp缩小在4B基因组上的154 766 751-250 584 662区域。【结论】矮秆波兰小麦与中国春具有一定的遗传差异。SLAF-Seq-BSA能有效地用于矮秆波兰小麦矮化基因Rht-dp的定位,为后期建立其他分子标记快速缩小目的区域奠定了物质基础。【 Objective】 Fine mapping of Rht-dp derived from dwarf polish wheat （ DPW）.【 Method】 In this study^ SLAF-Seq-BSA （ specific-locus amplified fragment sequencing and bulked segregant analysis） wasusedtomapthe Rht-dp associatedwithdwarfisminDPW.【 Results】 MostofSLAFtagswereevenly distributed in A and B genomes. However, a handful of tags were distributed in D genome or were not mapped on the genomes of Chinese Spring. Meanwhile, Rht-dp was mapped on the 4B genome started from 154 766 751 to 343 858 300. Combined with our previous SSR results Rht-dp was finally mapped on the 4B genome started from 154 766 751 to 250 584 662.【 Conclusion】 All results indicated that there are some differences of genetic similarity between DPW and Chinese Spring. SLAF-Seq -B SA is an efficient strategy for mapping the Rht-dpywhich would further help to fine map the Rht-dp by develo-ping other new molecular markers, such as SSR marker.

关 键 词：矮秆波兰小麦 SLAF-Seq BSA Rht-dp 

分 类 号：S512.5[农业科学—作物学]