猪流行性腹泻病毒HB-XL株S基因遗传变异分析  

Genetic variation analysis of Sgene of porcine epidemic diarrhea virus HB-XL strain

作　　者：李雅楠[1] 庞琳琳 李定刚[2] 李睿文[1,3] 孙继国 袁万哲[1,3] 

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]保定冀中药业有限公司,河北保定071100 [3]河北省兽医生物技术工程技术研究中心,河北保定071001

出　　处：《河北农业大学学报》2017年第6期93-96,共4页Journal of Hebei Agricultural University

基　　金：河北省高校百名优秀创新人才支持计划(Ⅲ)(SLRC2017039)

摘　　要：本研究从河北保定某猪场的粪便中分离到1株病毒,该病毒能在Vero细胞上增殖,且盲传5代后出现明显的细胞病变,经鉴定证实该分离毒株为猪流行性腹泻病毒(PEDV),并命名为HB-LX,应用DNAStar软件对HB-LX毒株的S基因进行遗传变异分析。结果表明,HB-LX与疫苗株CV777的同源性为92.4%,与野毒株MK、83P-5的同源性分别为92.2%、92.7%,与中国流行株同源性高达97%以上。HB-LX与我国CHCCC、GJL、PJ1407等和韩国的AS01、KPV1402等亲缘关系最近。In this study,a virus was isolated from the feces of a pig farm in Baoding,Hebei Province,which could proliferate on Vero cells and showed significant cytopathic changes after fifth passages.The isolate strain was identified and sequenced to be PEDV and named HBLX.Genetic analysis of Sgene of HB-LX strain was analyzed by DNA Star software.The results showed that the HB-LX shared 92.4% identities with vaccine strains CV777,shared92.2%and 92.7%identities with the field strain MK and 83 P-5,and shared more than 97%identities with the current strains in China.HB-LX and China＇s CHCCC,GJL,PJ1407 and South Korea＇s AS01,KPV1402 and other genetic relationship recently.

关 键 词：PEDV S基因 分离鉴定 序列分析 

分 类 号：S851.3[农业科学—预防兽医学]