肝脏免疫的活体显微光学成像研究进展  

Advances in Optical Microscopic Imaging for Visualizing Liver Immunology In vivo

作　　者：林巧雅[1,2] 黄松林 骆清铭 张智红[1,2] LIN Qiao-Ya;HUANG Song-Lin;LUO Qing-Ming;ZHANG Zhi-Hong(Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1Vuhan 430074, China;MOE Key Laboratory for Biomedical Phatonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Scienee and Technology, Wuhan 430074, China)

机构地区：[1]华中科技大学-武汉光电国家研究中心、Britton Chance生物医学光子学研究中心,武汉430074 [2]华中科技大学工程科学学院、生物医学光子学教育部重点实验室、生物医学工程协同创新中心,武汉430074

出　　处：《生物化学与生物物理进展》2017年第12期1056-1065,共10页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金重大研究计划(91542000,91442201);国家自然科学基金(81501593);国家杰出青年科学基金(81625012);中国博士后科学基金(2015M572148)资助项目~~

摘　　要：光学分子成像技术是在活体复杂的组织区域环境内细胞形态、运动与功能研究的最佳手段之一,极大地推进了免疫学的发展.肝脏是机体新陈代谢和解毒的重要器官,也被视为一个免疫器官.解析肝脏免疫基本特性和功能,对防治肝脏疾病以及全身性相关疾病具有重要意义.活体可视化研究肝脏区域生理或者病理状态下免疫应答,提供关键事件的多细胞参与及其彼此交互的时空动态信息,能极大地丰富对肝脏独特免疫反应的认知.本文将重点阐述目前活体肝脏成像的技术与方法以及光学显微成像技术,例如多光子激发显微成像与转盘共聚焦成像在肝脏免疫中的应用,并展望活体肝脏成像今后的发展方向和面临的机遇与挑战.Optical molecular imaging, which greatly promoted the development of immunology, is one of the best methods to investigate cell morphology, movement and function in a complex regional environment in vivo. The liver is a vital organ for maintaining body＇s metabolism and detoxification, and it as an immunological organ.Understanding the basic feature and function of liver immunology is significant for prevention and treatment of liver disease and associated systemic diseases. Visualization immune response under physiological or pathological condition in vivo, providing spatial-temporal and dynamic information of multiple cells involved in key events and their interactions, can greatly enrich the knowledge of liver unique immune response. This review will focus on the current liver intravital imaging techniques and methods, and the application of optical microscopic imaging techniques in liver immunology, such as multiphoton excitation confocal microscopy and spin-disk confocal microscopy, and future development directions and challenges of in vivo liver imaging are prospected.

关 键 词：多光子激发显微成像 转盘共聚焦成像 活体腹窗 肿瘤肝转移 急性肝损伤 局灶性肝损伤和炎症 

分 类 号：O43[机械工程—光学工程] R392[理学—光学]