长白白眉蝮蛇类凝血酶的研究Ⅰ——纯化方法的研究  

STUDY ON PURIFICATION OF A THROXBN-LIKE E ZYME FROM VENOM OF AGKISTRODON HALYS USSURIENSIS ENELIANOV

作　　者：王晓辉[1] 何宝俊 

机构地区：[1]中国医科大学医学基础部化学检测中心实验室

出　　处：《中国医科大学学报》1991年第2期114-120,共7页Journal of China Medical University

摘　　要：采用四种纯化方法,对长白白眉蝮蛇类凝血酶的纯化途径进行研究。结果,阴阳离子交换柱、分子筛柱层析法周期较长,收率较低。羟基磷灰石柱法。对类凝血酶有较好的分离效果,但要求样品量小且纯度较高。亲和层析法分离类凝血酶,具有操作简便、纯化周期短、回收率高、处理样品量大等特点。A thrombin-like enzyme has been purified homogeneitically by SDS-PAGEfrom the venom of Agkistrodon halys ussuriensis Emelianov by mean of home-made affinity column on fast protein liquid chromatography system. Thismethod is rapider and more convenient and high over-all recovery than bymeans of ion-exchange chromatography and gel filtration chromatography.

关 键 词：长白白眉蝮蛇 类凝血酶 纯化 

分 类 号：R284.2[医药卫生—中药学]