榉木尘提取液对小鼠肺组织脂质过氧化及ref-1基因表达的影响  

Effects of beech dust extract on lipid peroxidation and expression of ref-1 gene of mouse lung tissue

作　　者：何鹏[1] 吕晓华[2] 吴德生[3] 董奇男[3] 王瑞淑[3] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京100050 [2]四川大学生命科学学院生物工程研究所 [3]四川大学公共卫生学院

出　　处：《卫生研究》2002年第4期217-219,共3页Journal of Hygiene Research

摘　　要：为观察榉木尘提取液对小鼠肺组织脂质过氧化和氧化还原相关基因ref 1表达的影响 ,采用榉木尘水提取液和有机提取液建立小鼠肺肿瘤短期诱发实验二阶段模型 ,将染毒小鼠处死后分为非荷瘤肺组织组、肺乳头状腺瘤组织组、肺腺瘤组织组 ,将溶剂组设为对照组 ,TAB法检测肺组织匀浆脂质过氧化物 ,免疫组织化学染色法检测肿瘤组织中ref 1基因的表达。结果显示 :①所有染毒组肺组织中TBARS显著高于溶剂对照组 ,其中 ,肺乳头状腺瘤组、肺腺瘤组 (P <0 0 1) ,染毒的非荷瘤肺组织组 (P <0 0 5 ) ;②溶剂对照组和染毒的正常肺组织组Ref 1免疫组织化学染色反应为阴性 ,肺乳头状腺瘤表现为强烈的核着色 ,肺腺瘤则表现为核和胞浆着色。提示榉木尘的毒作用机制可能与其改变Ref 1的氧化还原调控功能 。In order to explore the effects of beech dust on lipid peroxidation and expression of redox-related gene ref-1, the 2-stage model of mouse lung tumor short-term induction test is used. The mice exposed to the extract beech dust are divided into 3 groups(group of normal lung tissue, group of papillary adenoma and group of lung adenoma)with the mice treated with solvents as control. The extent of homogenate lipid peroxides is determined by measuring the formation of TBARS, and expression of ref-1 is measured by immunohistochemistry. The results show that ①The formation of TBARS of all groups treated with the beech extracts of beech dust is increased compared with the control, and the differences between the groups with adenoma and the control are statistically significant (P<0.01), while the P value of that of the group of normal lung tissue is less than 0.05. ②Both the control and the group of normal lung tissue treated with the extracts are Ref-1-negative, strong nuclear Ref-1 immunostaining is found in the group of papillary adenoma, and the cytoplasmic/ nuclear expression of Ref-1 is significantly enhanced in the group of lung adenoma and group of mixed adenoma. It is suggested that the extracts of beech dust toxicity is probably related to its altering redox regulation of ref-1 and subsequent disturbance of redox status.

关 键 词：提取液 肺组织 脂质过氧化 榉木尘 脂质过氧化 REF-1 

分 类 号：R285.5[医药卫生—中药学]