HPLC法同时测定腰痛胶囊中4种成分的含量  

Simultaneous Determination of 4 Constituents in Yaotong Capsules by HPLC

作　　者：罗春颖 黄晓婧 史立川 蒲旭峰 

机构地区：[1]成都中医药大学药学院,成都611137 [2]成都市食品药品检验研究院,成都610000

出　　处：《中国药房》2017年第36期5123-5126,共4页China Pharmacy

基　　金：国家药品标准提高研究课题(No.2015-26)

摘　　要：目的:建立同时测定腰痛胶囊中芍药苷、桂皮醛、补骨脂素、异补骨脂素含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent-C18,流动相为乙腈-水(梯度洗脱),流速为1.0 mL/min,检测波长为240 nm(芍药苷、补骨脂素、异补骨脂素)、290 nm(桂皮醛),柱温为35℃,进样量为10μL。结果:芍药苷、桂皮醛、补骨脂素、异补骨脂素检测质量浓度线性范围分别为0.004 02~0.100 6 mg/mL(r=0.999 8)、0.024 50~0.612 7 mg/mL(r=0.999 8)、0.005 24~0.131 0 mg/mL(r=0.999 9)、0.005 11~0.127 8mg/mL(r=0.999 9);定量限分别为37.17、1.47、21.76、25.57 ng,检测限分别为11.15、0.44、6.53、7.67 ng;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为99.02%~100.83%(RSD=0.68%,n=6)、98.58%~100.99%(RSD=0.83%,n=6)、99.78%~101.69%(RSD=0.89%,n=6)、100.06%~102.46%(RSD=0.92%,n=6)。结论:该方法操作简单、结果准确,适用于腰痛胶囊中芍药苷、桂皮醛、补骨脂素、异补骨脂素含量的同时测定。OBJECTIVE： To establish a method for the simultaneous determination of paeoniflorin, cinnamaldehyde, psoralen and isopsoralen in Yaotong capsules. METHODS ： HPLC method was adopted. The determination was performed on Agilent-C18 col- umn with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelengths were set at 240 nm （paeoniflorin, psoralen, isopsoralen） and 290 nm （cinnamaldehyde）. The column temperature was 35 ℃, and sample size was 10 μL. RESULTS： The linear ranges of paeoniflorin, cirmamaldehyde, psoralen and isopsoralen were 0.004 02- 0.100 6 mg/mL（r=0.999 8）, 0.024 50-0.612 7 mg/mL（r=0.999 8）,0.005 24-0.131 0 mg/mL（r=0.999 9）,0.005 11-0.127 8 mg/mL（r=0.999 9）, respectively. LOQ were 37.17, 1.47, 21.76, 25.57 ng, respectively; LOD were 11.15, 0.44, 6.53, 7.67 ng, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 99.02%-100.83% （RSD=0.68%, n=6）, 98.58% -100.99% （RSD=0.83%, n=6）, 99.78% -101.69% （RSD=0.89%, n=6）, 100.06% -102.46% （RSD=0.92%, n=6）, respectively. CONCLUSIONS： The method is simple, accurate and suitable for simultaneous determination of paeoniflorin, cinnamaldehyde, psoralen and isopsoralen in Yaotong capsules.

关 键 词：腰痛胶囊 芍药苷 桂皮醛 补骨脂素 异补骨脂素 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]